miRNA display CGI


Results 1 - 6 of 6 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
22187 5' -57.8 NC_004927.1 + 65821 0.66 0.687255
Target:  5'- aCCGCGuuaUCCGCGACaugaaGCGGGa -3'
miRNA:   3'- -GGUGUug-AGGCGCUGgagcaCGCCCa -5'
22187 5' -57.8 NC_004927.1 + 38673 0.67 0.645567
Target:  5'- -----uUUCCGCGACCUCGgcagaGCaGGGUu -3'
miRNA:   3'- gguguuGAGGCGCUGGAGCa----CG-CCCA- -5'
22187 5' -57.8 NC_004927.1 + 25524 0.68 0.593263
Target:  5'- aCCACGucuuCUUCGcCGcCCUCGgagaGCGGGa -3'
miRNA:   3'- -GGUGUu---GAGGC-GCuGGAGCa---CGCCCa -5'
22187 5' -57.8 NC_004927.1 + 51181 0.68 0.593263
Target:  5'- cCCACAAUUCCGUGugCggauuaGU-CGGGa -3'
miRNA:   3'- -GGUGUUGAGGCGCugGag----CAcGCCCa -5'
22187 5' -57.8 NC_004927.1 + 35189 0.68 0.593263
Target:  5'- gCGuCGGCUcCCGUGAaguCCUCG-GCGGGg -3'
miRNA:   3'- gGU-GUUGA-GGCGCU---GGAGCaCGCCCa -5'
22187 5' -57.8 NC_004927.1 + 56145 0.69 0.531541
Target:  5'- -gGCGGCUcCCGaCGAcCCUCGUGUGuGGa -3'
miRNA:   3'- ggUGUUGA-GGC-GCU-GGAGCACGC-CCa -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.