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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio #
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P value |
| Predicted miRNA align pattern | |||||||
| 22188 | 5' | -49.8 | NC_004927.1 | + | 65353 | 0.66 | 0.977277 |
|
Target: 5'- cGGCUUcCAaGCUCGAAGAagGCGAc- -3' miRNA: 3'- -CCGGAaGUaCGAGCUUCUggUGUUag -5' |
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| 22188 | 5' | -49.8 | NC_004927.1 | + | 61165 | 0.66 | 0.977277 |
|
Target: 5'- uGGCCUccgCGcgaaGCUCGGAaucacGACCACGccaAUCa -3' miRNA: 3'- -CCGGAa--GUa---CGAGCUU-----CUGGUGU---UAG- -5' |
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| 22188 | 5' | -49.8 | NC_004927.1 | + | 33090 | 0.71 | 0.789742 |
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Target: 5'- cGCCguggUC-UGCUCGAAGuCCAgacCAAUCg -3' miRNA: 3'- cCGGa---AGuACGAGCUUCuGGU---GUUAG- -5' |
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| 22188 | 5' | -49.8 | NC_004927.1 | + | 34981 | 0.71 | 0.799678 |
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Target: 5'- aGCCUgaaUAUGCUCGAAcgggccgaaguGACCACGAc- -3' miRNA: 3'- cCGGAa--GUACGAGCUU-----------CUGGUGUUag -5' |
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| 22188 | 5' | -49.8 | NC_004927.1 | + | 40537 | 0.73 | 0.694048 |
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Target: 5'- cGGCCUcaaCAUGgUCGAAGAcuaCCACGAcuUCa -3' miRNA: 3'- -CCGGAa--GUACgAGCUUCU---GGUGUU--AG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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