miRNA display CGI


Results 1 - 5 of 5 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
22188 5' -49.8 NC_004927.1 + 40537 0.73 0.694048
Target:  5'- cGGCCUcaaCAUGgUCGAAGAcuaCCACGAcuUCa -3'
miRNA:   3'- -CCGGAa--GUACgAGCUUCU---GGUGUU--AG- -5'
22188 5' -49.8 NC_004927.1 + 33090 0.71 0.789742
Target:  5'- cGCCguggUC-UGCUCGAAGuCCAgacCAAUCg -3'
miRNA:   3'- cCGGa---AGuACGAGCUUCuGGU---GUUAG- -5'
22188 5' -49.8 NC_004927.1 + 34981 0.71 0.799678
Target:  5'- aGCCUgaaUAUGCUCGAAcgggccgaaguGACCACGAc- -3'
miRNA:   3'- cCGGAa--GUACGAGCUU-----------CUGGUGUUag -5'
22188 5' -49.8 NC_004927.1 + 65353 0.66 0.977277
Target:  5'- cGGCUUcCAaGCUCGAAGAagGCGAc- -3'
miRNA:   3'- -CCGGAaGUaCGAGCUUCUggUGUUag -5'
22188 5' -49.8 NC_004927.1 + 61165 0.66 0.977277
Target:  5'- uGGCCUccgCGcgaaGCUCGGAaucacGACCACGccaAUCa -3'
miRNA:   3'- -CCGGAa--GUa---CGAGCUU-----CUGGUGU---UAG- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.