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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 22193 | 5' | -54.6 | NC_004927.1 | + | 7657 | 0.66 | 0.842385 |
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Target: 5'- uUCGcacAGCUCCGGCAGuAGUaucGCuuGUUCg -3' miRNA: 3'- -AGU---UCGGGGUUGUCuUCA---CGggCAGG- -5' |
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| 22193 | 5' | -54.6 | NC_004927.1 | + | 20319 | 0.66 | 0.841527 |
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Target: 5'- --cGGCCCCAcCGGAGGgaGCgucgucgUCGUCCa -3' miRNA: 3'- aguUCGGGGUuGUCUUCa-CG-------GGCAGG- -5' |
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| 22193 | 5' | -54.6 | NC_004927.1 | + | 56385 | 0.66 | 0.815749 |
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Target: 5'- aCAAGCCCgCAGagacCAGAccgGCUCGUUCg -3' miRNA: 3'- aGUUCGGG-GUU----GUCUucaCGGGCAGG- -5' |
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| 22193 | 5' | -54.6 | NC_004927.1 | + | 29373 | 0.7 | 0.608932 |
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Target: 5'- -gAGGCCCCAACAGguGUGgcacUUCGUCg -3' miRNA: 3'- agUUCGGGGUUGUCuuCAC----GGGCAGg -5' |
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| 22193 | 5' | -54.6 | NC_004927.1 | + | 65621 | 0.71 | 0.54474 |
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Target: 5'- -gAAGCCCCAcacGCAGggGcGCUCGacgcugguguUCCa -3' miRNA: 3'- agUUCGGGGU---UGUCuuCaCGGGC----------AGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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