Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 22194 | 5' | -46.6 | NC_004927.1 | + | 63490 | 0.66 | 0.998086 |
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Target: 5'- cGGAGAGCUGA-CgGAaguggggacguugAAAUCUACGc -3' miRNA: 3'- uCCUCUCGAUUaGaCU-------------UUUAGGUGCu -5' |
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| 22194 | 5' | -46.6 | NC_004927.1 | + | 34395 | 0.66 | 0.997251 |
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Target: 5'- cGGuGAGCUGGUCgcUGAGGucGUCCAg-- -3' miRNA: 3'- uCCuCUCGAUUAG--ACUUU--UAGGUgcu -5' |
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| 22194 | 5' | -46.6 | NC_004927.1 | + | 67290 | 0.67 | 0.992447 |
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Target: 5'- gAGGAGGGCaagGAgacgGAAGAccgcaucaUCCACGAu -3' miRNA: 3'- -UCCUCUCGa--UUaga-CUUUU--------AGGUGCU- -5' |
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| 22194 | 5' | -46.6 | NC_004927.1 | + | 3974 | 0.69 | 0.982548 |
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Target: 5'- aAGGAGuguGGCUucuucauaggcAGUCUGAGuAUCCGCa- -3' miRNA: 3'- -UCCUC---UCGA-----------UUAGACUUuUAGGUGcu -5' |
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| 22194 | 5' | -46.6 | NC_004927.1 | + | 52609 | 0.69 | 0.980227 |
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Target: 5'- cGGGAGAGgaAAUCUcGAAAaucGUCCA-GAa -3' miRNA: 3'- -UCCUCUCgaUUAGA-CUUU---UAGGUgCU- -5' |
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| 22194 | 5' | -46.6 | NC_004927.1 | + | 14602 | 0.71 | 0.937922 |
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Target: 5'- cGuGGGAGC-GGUCUGGAGcguUCCACGGc -3' miRNA: 3'- uC-CUCUCGaUUAGACUUUu--AGGUGCU- -5' |
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| 22194 | 5' | -46.6 | NC_004927.1 | + | 75235 | 0.72 | 0.91387 |
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Target: 5'- cGGGGAGCUGGUC-GAGccAAUCCGgguCGAu -3' miRNA: 3'- uCCUCUCGAUUAGaCUU--UUAGGU---GCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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