Results 41 - 43 of 43 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22233 | 5' | -61.6 | NC_005045.1 | + | 29992 | 0.66 | 0.312477 |
Target: 5'- aGGCGGCCaacgGCUCaAGCCugacGGAUCGCCu -3' miRNA: 3'- cUCGUCGG----UGGG-UCGGu---CCUGGUGGu -5' |
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22233 | 5' | -61.6 | NC_005045.1 | + | 7502 | 0.66 | 0.312477 |
Target: 5'- uAGCAguauGCCACCCAGCgguCAGGAagguuCUugCAg -3' miRNA: 3'- cUCGU----CGGUGGGUCG---GUCCU-----GGugGU- -5' |
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22233 | 5' | -61.6 | NC_005045.1 | + | 32182 | 0.66 | 0.312477 |
Target: 5'- cAGCAGCCAUgCCAGCac-GACCgaGCCGc -3' miRNA: 3'- cUCGUCGGUG-GGUCGgucCUGG--UGGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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