Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 22234 | 5' | -57 | NC_005045.1 | + | 18686 | 0.66 | 0.554635 |
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Target: 5'- aCCUGGGGCgg-CCCugGCc---GGAg -3' miRNA: 3'- gGGACCCCGgaaGGGugCGcauaUCU- -5' |
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| 22234 | 5' | -57 | NC_005045.1 | + | 40121 | 0.67 | 0.474805 |
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Target: 5'- cCCUUGaGGGCCUUguccgcgagcuagcaCCCACGCucGUGaAGGa -3' miRNA: 3'- -GGGAC-CCCGGAA---------------GGGUGCG--CAUaUCU- -5' |
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| 22234 | 5' | -57 | NC_005045.1 | + | 32627 | 0.67 | 0.470756 |
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Target: 5'- gCCUGGGGCagcguaCCGCGUuccGUGUGGu -3' miRNA: 3'- gGGACCCCGgaag--GGUGCG---CAUAUCu -5' |
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| 22234 | 5' | -57 | NC_005045.1 | + | 41534 | 0.68 | 0.460712 |
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Target: 5'- gCCUUGGccuugaaucGGCCUcgUCCAUGuCGUGUAGGg -3' miRNA: 3'- -GGGACC---------CCGGAa-GGGUGC-GCAUAUCU- -5' |
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| 22234 | 5' | -57 | NC_005045.1 | + | 30024 | 0.68 | 0.450782 |
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Target: 5'- cCCaCUGcuccaGGGCCUcagugacguucgUCCCcaGCGUGUGUAGGg -3' miRNA: 3'- -GG-GAC-----CCCGGA------------AGGG--UGCGCAUAUCU- -5' |
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| 22234 | 5' | -57 | NC_005045.1 | + | 30052 | 0.68 | 0.421718 |
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Target: 5'- gCCUGGGGaCCUUCCCcaagaccuacgGCGaGUccGUGGGc -3' miRNA: 3'- gGGACCCC-GGAAGGG-----------UGCgCA--UAUCU- -5' |
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| 22234 | 5' | -57 | NC_005045.1 | + | 590 | 0.68 | 0.412283 |
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Target: 5'- uCCCUGGGuGCUggucuaccUCCCugGCGcUGUGc- -3' miRNA: 3'- -GGGACCC-CGGa-------AGGGugCGC-AUAUcu -5' |
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| 22234 | 5' | -57 | NC_005045.1 | + | 8104 | 0.72 | 0.234047 |
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Target: 5'- uCCUUGGGGUCgaacccgUCCgGCGUGUGUAu- -3' miRNA: 3'- -GGGACCCCGGa------AGGgUGCGCAUAUcu -5' |
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| 22234 | 5' | -57 | NC_005045.1 | + | 926 | 1.1 | 0.000409 |
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Target: 5'- uCCCUGGGGCCUUCCCACGCGUAUAGAg -3' miRNA: 3'- -GGGACCCCGGAAGGGUGCGCAUAUCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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