Results 1 - 7 of 7 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22241 | 5' | -57.2 | NC_005045.1 | + | 237 | 0.66 | 0.502852 |
Target: 5'- -cUGGCUgcuggguaCGGGCUGUGCUGCUCa-- -3' miRNA: 3'- gaAUCGGa-------GCCCGACGUGAUGGGcua -5' |
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22241 | 5' | -57.2 | NC_005045.1 | + | 42300 | 0.66 | 0.502852 |
Target: 5'- -cUGGCUgcuggguaCGGGCUGUGCUGCUCa-- -3' miRNA: 3'- gaAUCGGa-------GCCCGACGUGAUGGGcua -5' |
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22241 | 5' | -57.2 | NC_005045.1 | + | 18820 | 0.66 | 0.481812 |
Target: 5'- uCUgcGCCUUGGcGCUGUAgUAUCCGc- -3' miRNA: 3'- -GAauCGGAGCC-CGACGUgAUGGGCua -5' |
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22241 | 5' | -57.2 | NC_005045.1 | + | 37089 | 0.68 | 0.393036 |
Target: 5'- -gUAGUCUCuGaGCUGUACU-CCCGAUc -3' miRNA: 3'- gaAUCGGAGcC-CGACGUGAuGGGCUA- -5' |
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22241 | 5' | -57.2 | NC_005045.1 | + | 24045 | 0.68 | 0.383842 |
Target: 5'- --aAGCCgauauGGCUGCGCUGCgCGAg -3' miRNA: 3'- gaaUCGGagc--CCGACGUGAUGgGCUa -5' |
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22241 | 5' | -57.2 | NC_005045.1 | + | 33024 | 0.68 | 0.357132 |
Target: 5'- -gUAGCCUgGGGcCUGaCGgaUACCCGAUu -3' miRNA: 3'- gaAUCGGAgCCC-GAC-GUg-AUGGGCUA- -5' |
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22241 | 5' | -57.2 | NC_005045.1 | + | 4427 | 1.06 | 0.000617 |
Target: 5'- gCUUAGCCUCGGGCUGCACUACCCGAUa -3' miRNA: 3'- -GAAUCGGAGCCCGACGUGAUGGGCUA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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