Results 1 - 20 of 24 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22243 | 3' | -59 | NC_005045.1 | + | 16757 | 0.66 | 0.443823 |
Target: 5'- cCugGCCGaCCACg-UUGCCCAcGG-CGc -3' miRNA: 3'- uGugCGGC-GGUGgaAACGGGU-CCaGC- -5' |
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22243 | 3' | -59 | NC_005045.1 | + | 11495 | 0.66 | 0.443823 |
Target: 5'- cCACGCCGUCgagguugaACCagcggUUGUUCGGGUCu -3' miRNA: 3'- uGUGCGGCGG--------UGGa----AACGGGUCCAGc -5' |
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22243 | 3' | -59 | NC_005045.1 | + | 38058 | 0.66 | 0.434145 |
Target: 5'- gGCGCuGCCcucugGCCGCCgaucGUCUAGGUCu -3' miRNA: 3'- -UGUG-CGG-----CGGUGGaaa-CGGGUCCAGc -5' |
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22243 | 3' | -59 | NC_005045.1 | + | 8695 | 0.66 | 0.434145 |
Target: 5'- -gGCGCUGCCACCagcaccgagGCCCcGG-CGu -3' miRNA: 3'- ugUGCGGCGGUGGaaa------CGGGuCCaGC- -5' |
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22243 | 3' | -59 | NC_005045.1 | + | 14316 | 0.66 | 0.432224 |
Target: 5'- uCACGUCGCC-CCacagugcgaaGCCCAGG-CGg -3' miRNA: 3'- uGUGCGGCGGuGGaaa-------CGGGUCCaGC- -5' |
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22243 | 3' | -59 | NC_005045.1 | + | 17815 | 0.66 | 0.428397 |
Target: 5'- uGCAaugGCCGCCGCCgaggugGCCCugaccgaggaggcuGGUCc -3' miRNA: 3'- -UGUg--CGGCGGUGGaaa---CGGGu-------------CCAGc -5' |
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22243 | 3' | -59 | NC_005045.1 | + | 5806 | 0.66 | 0.415162 |
Target: 5'- aGCugGCCGCUACCcgcGCCUcuAGaUCGg -3' miRNA: 3'- -UGugCGGCGGUGGaaaCGGG--UCcAGC- -5' |
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22243 | 3' | -59 | NC_005045.1 | + | 40407 | 0.66 | 0.415162 |
Target: 5'- cCACGCCGgUgacggcgaggguGCCUUUGCCCGccUCGg -3' miRNA: 3'- uGUGCGGCgG------------UGGAAACGGGUccAGC- -5' |
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22243 | 3' | -59 | NC_005045.1 | + | 19412 | 0.66 | 0.405864 |
Target: 5'- aGCGCGCCcUCGCCgaagGCCCggagaacuauccGGGUCu -3' miRNA: 3'- -UGUGCGGcGGUGGaaa-CGGG------------UCCAGc -5' |
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22243 | 3' | -59 | NC_005045.1 | + | 30601 | 0.66 | 0.404941 |
Target: 5'- --uCGCCGCCGCCaacgUGCgcaaccgcaccauCCAGGUgGg -3' miRNA: 3'- uguGCGGCGGUGGaa--ACG-------------GGUCCAgC- -5' |
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22243 | 3' | -59 | NC_005045.1 | + | 32683 | 0.67 | 0.387666 |
Target: 5'- gUACGCUGCC-CCag-GCCCuccaGGGUCu -3' miRNA: 3'- uGUGCGGCGGuGGaaaCGGG----UCCAGc -5' |
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22243 | 3' | -59 | NC_005045.1 | + | 39698 | 0.67 | 0.387666 |
Target: 5'- aGCuCGUCGCCGCCgucGCCggcaGGGUCc -3' miRNA: 3'- -UGuGCGGCGGUGGaaaCGGg---UCCAGc -5' |
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22243 | 3' | -59 | NC_005045.1 | + | 2461 | 0.67 | 0.370013 |
Target: 5'- gGCA-GCCGCCGCaa-UGCgCuGGUCGa -3' miRNA: 3'- -UGUgCGGCGGUGgaaACGgGuCCAGC- -5' |
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22243 | 3' | -59 | NC_005045.1 | + | 2766 | 0.67 | 0.370013 |
Target: 5'- cCAUGCCGacgauCACC-UUGCCCuuGGUCu -3' miRNA: 3'- uGUGCGGCg----GUGGaAACGGGu-CCAGc -5' |
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22243 | 3' | -59 | NC_005045.1 | + | 28156 | 0.68 | 0.344581 |
Target: 5'- aGCGCugugguGCCGCUGCCg--GCaCGGGUCGa -3' miRNA: 3'- -UGUG------CGGCGGUGGaaaCGgGUCCAGC- -5' |
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22243 | 3' | -59 | NC_005045.1 | + | 29956 | 0.68 | 0.342108 |
Target: 5'- -aGgGCCGCCAugaugcCCUgcaugcguugggcaUUGCCCAGGcCGg -3' miRNA: 3'- ugUgCGGCGGU------GGA--------------AACGGGUCCaGC- -5' |
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22243 | 3' | -59 | NC_005045.1 | + | 41583 | 0.68 | 0.32043 |
Target: 5'- -aGCGCCGCCcUCgccGCCCAGGg-- -3' miRNA: 3'- ugUGCGGCGGuGGaaaCGGGUCCagc -5' |
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22243 | 3' | -59 | NC_005045.1 | + | 37389 | 0.69 | 0.29757 |
Target: 5'- -aGCGCCGCCGCCgg-GaCCGGGa-- -3' miRNA: 3'- ugUGCGGCGGUGGaaaCgGGUCCagc -5' |
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22243 | 3' | -59 | NC_005045.1 | + | 25840 | 0.69 | 0.288064 |
Target: 5'- -uGCGCCGCCACCggcugaagcuacgcUUgGCCUGGGUg- -3' miRNA: 3'- ugUGCGGCGGUGG--------------AAaCGGGUCCAgc -5' |
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22243 | 3' | -59 | NC_005045.1 | + | 17922 | 0.69 | 0.283045 |
Target: 5'- cCGCGCCGCCACCcugGaaCAGG-CGg -3' miRNA: 3'- uGUGCGGCGGUGGaaaCggGUCCaGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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