Results 21 - 24 of 24 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22243 | 3' | -59 | NC_005045.1 | + | 38058 | 0.66 | 0.434145 |
Target: 5'- gGCGCuGCCcucugGCCGCCgaucGUCUAGGUCu -3' miRNA: 3'- -UGUG-CGG-----CGGUGGaaa-CGGGUCCAGc -5' |
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22243 | 3' | -59 | NC_005045.1 | + | 39698 | 0.67 | 0.387666 |
Target: 5'- aGCuCGUCGCCGCCgucGCCggcaGGGUCc -3' miRNA: 3'- -UGuGCGGCGGUGGaaaCGGg---UCCAGc -5' |
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22243 | 3' | -59 | NC_005045.1 | + | 40407 | 0.66 | 0.415162 |
Target: 5'- cCACGCCGgUgacggcgaggguGCCUUUGCCCGccUCGg -3' miRNA: 3'- uGUGCGGCgG------------UGGAAACGGGUccAGC- -5' |
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22243 | 3' | -59 | NC_005045.1 | + | 41583 | 0.68 | 0.32043 |
Target: 5'- -aGCGCCGCCcUCgccGCCCAGGg-- -3' miRNA: 3'- ugUGCGGCGGuGGaaaCGGGUCCagc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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