miRNA: 3'- -UGUGCGGCGGUGGAAacGGGUCCAGC- -5'
miRNA: 3'- -UGugCGGCGGUGGaaaCGGG--UCcAGC- -5'
miRNA: 3'- uGUGCGGCg----GUGGaAACGGGu-CCAGc -5'
miRNA: 3'- -UGUgCGGCGGUGgaaACGgGuCCAGC- -5'
Results 21 - 24 of 24 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 22243 | 3' | -59 | NC_005045.1 | + | 6545 | 0.93 | 0.004936 |
|
Target: 5'- cACACGCCGCCACCUUguCCCAGGUCGc -3' miRNA: 3'- -UGUGCGGCGGUGGAAacGGGUCCAGC- -5' |
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| 22243 | 3' | -59 | NC_005045.1 | + | 5806 | 0.66 | 0.415162 |
|
Target: 5'- aGCugGCCGCUACCcgcGCCUcuAGaUCGg -3' miRNA: 3'- -UGugCGGCGGUGGaaaCGGG--UCcAGC- -5' |
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| 22243 | 3' | -59 | NC_005045.1 | + | 2766 | 0.67 | 0.370013 |
|
Target: 5'- cCAUGCCGacgauCACC-UUGCCCuuGGUCu -3' miRNA: 3'- uGUGCGGCg----GUGGaAACGGGu-CCAGc -5' |
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| 22243 | 3' | -59 | NC_005045.1 | + | 2461 | 0.67 | 0.370013 |
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Target: 5'- gGCA-GCCGCCGCaa-UGCgCuGGUCGa -3' miRNA: 3'- -UGUgCGGCGGUGgaaACGgGuCCAGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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