Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 22251 | 3' | -56.8 | NC_005045.1 | + | 5666 | 0.66 | 0.530863 |
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Target: 5'- uUCUugggCGUAUaGGCgugggcgaauGCCCGGCUCUUGg- -3' miRNA: 3'- -AGAa---GCAUG-CCG----------UGGGCCGGGAACau -5' |
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| 22251 | 3' | -56.8 | NC_005045.1 | + | 40348 | 0.67 | 0.445664 |
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Target: 5'- gUCUUCGUccuuuuggGCGGCccuugcgacgcuCCCGGCCCa---- -3' miRNA: 3'- -AGAAGCA--------UGCCGu-----------GGGCCGGGaacau -5' |
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| 22251 | 3' | -56.8 | NC_005045.1 | + | 31724 | 0.68 | 0.437756 |
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Target: 5'- --aUCGUAgCGGaccaggaaCugCCGGCCCUUGa- -3' miRNA: 3'- agaAGCAU-GCC--------GugGGCCGGGAACau -5' |
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| 22251 | 3' | -56.8 | NC_005045.1 | + | 4779 | 0.68 | 0.408839 |
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Target: 5'- gUCUUCG-ACGGUGgCCGGCUuCUUGa- -3' miRNA: 3'- -AGAAGCaUGCCGUgGGCCGG-GAACau -5' |
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| 22251 | 3' | -56.8 | NC_005045.1 | + | 24416 | 0.72 | 0.248985 |
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Target: 5'- cUCUUCac-CGGCACgaCGGCCCUUGg- -3' miRNA: 3'- -AGAAGcauGCCGUGg-GCCGGGAACau -5' |
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| 22251 | 3' | -56.8 | NC_005045.1 | + | 12105 | 1.07 | 0.000658 |
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Target: 5'- aUCUUCGUACGGCACCCGGCCCUUGUAc -3' miRNA: 3'- -AGAAGCAUGCCGUGGGCCGGGAACAU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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