Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 22270 | 3' | -56.6 | NC_005045.1 | + | 26679 | 0.66 | 0.569362 |
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Target: 5'- -aCGGCGGAccUGcCGgCCUUGcUGCCGGg -3' miRNA: 3'- uaGCUGCCU--AC-GUgGGAACuGCGGCU- -5' |
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| 22270 | 3' | -56.6 | NC_005045.1 | + | 32403 | 0.66 | 0.555113 |
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Target: 5'- cUCGGCGGucauggcuacuucugGCAgCCCUacaguaGGCGCCGAu -3' miRNA: 3'- uAGCUGCCua-------------CGU-GGGAa-----CUGCGGCU- -5' |
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| 22270 | 3' | -56.6 | NC_005045.1 | + | 13565 | 0.66 | 0.525877 |
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Target: 5'- cUCGGCGGcgGUGUCCUUGugGCa-- -3' miRNA: 3'- uAGCUGCCuaCGUGGGAACugCGgcu -5' |
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| 22270 | 3' | -56.6 | NC_005045.1 | + | 31845 | 0.67 | 0.473412 |
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Target: 5'- -cCGGCGaGGUGgACCCUaacuucGACGCUGGg -3' miRNA: 3'- uaGCUGC-CUACgUGGGAa-----CUGCGGCU- -5' |
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| 22270 | 3' | -56.6 | NC_005045.1 | + | 5002 | 0.68 | 0.414148 |
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Target: 5'- uGUCGcugaaGCGGAccaGCACCUgauaGACGCCGGc -3' miRNA: 3'- -UAGC-----UGCCUa--CGUGGGaa--CUGCGGCU- -5' |
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| 22270 | 3' | -56.6 | NC_005045.1 | + | 32218 | 0.69 | 0.359672 |
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Target: 5'- -gUGGCGGGUGC--UCUUGGCGCUGGc -3' miRNA: 3'- uaGCUGCCUACGugGGAACUGCGGCU- -5' |
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| 22270 | 3' | -56.6 | NC_005045.1 | + | 5086 | 0.69 | 0.359672 |
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Target: 5'- cGUCGAgGGuUGCAgCCUgGGCuGCCGAc -3' miRNA: 3'- -UAGCUgCCuACGUgGGAaCUG-CGGCU- -5' |
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| 22270 | 3' | -56.6 | NC_005045.1 | + | 2815 | 0.76 | 0.131414 |
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Target: 5'- -gCGAUGGGUGCAgCCUUGGCgGCCa- -3' miRNA: 3'- uaGCUGCCUACGUgGGAACUG-CGGcu -5' |
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| 22270 | 3' | -56.6 | NC_005045.1 | + | 28628 | 1.07 | 0.000721 |
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Target: 5'- cAUCGACGGAUGCACCCUUGACGCCGAa -3' miRNA: 3'- -UAGCUGCCUACGUGGGAACUGCGGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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