miRNA display CGI


Results 1 - 7 of 7 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
22271 5' -56.1 NC_005045.1 + 36928 0.66 0.572582
Target:  5'- uGUCccUCgUGGcgCcuguggcuaaugccAGGGCCUCGCUGAa -3'
miRNA:   3'- -CAGuuAG-ACCuaG--------------UCCCGGAGCGGCU- -5'
22271 5' -56.1 NC_005045.1 + 38433 0.66 0.598171
Target:  5'- aUCGcgCUGGA---GGGCUucaUCGCCGGu -3'
miRNA:   3'- cAGUuaGACCUaguCCCGG---AGCGGCU- -5'
22271 5' -56.1 NC_005045.1 + 12454 0.67 0.53202
Target:  5'- cUCAAggcUCUGGAgaaaCAGGGCUUCGaggCGGc -3'
miRNA:   3'- cAGUU---AGACCUa---GUCCCGGAGCg--GCU- -5'
22271 5' -56.1 NC_005045.1 + 31666 0.67 0.49991
Target:  5'- cGUCGAgUUGGcccuGUacuuGGGCCUCGUCGAg -3'
miRNA:   3'- -CAGUUaGACC----UAgu--CCCGGAGCGGCU- -5'
22271 5' -56.1 NC_005045.1 + 39707 0.68 0.428681
Target:  5'- aUCGGcggCUGGAagGGuGGCUUCGCUGAg -3'
miRNA:   3'- cAGUUa--GACCUagUC-CCGGAGCGGCU- -5'
22271 5' -56.1 NC_005045.1 + 8540 0.68 0.478987
Target:  5'- -------cGGcgCAGGGCCUCGUCGc -3'
miRNA:   3'- caguuagaCCuaGUCCCGGAGCGGCu -5'
22271 5' -56.1 NC_005045.1 + 28682 1.09 0.000588
Target:  5'- cGUCAAUCUGGAUCAGGGCCUCGCCGAc -3'
miRNA:   3'- -CAGUUAGACCUAGUCCCGGAGCGGCU- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.