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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 22271 | 5' | -56.1 | NC_005045.1 | + | 39707 | 0.68 | 0.428681 |
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Target: 5'- aUCGGcggCUGGAagGGuGGCUUCGCUGAg -3' miRNA: 3'- cAGUUa--GACCUagUC-CCGGAGCGGCU- -5' |
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| 22271 | 5' | -56.1 | NC_005045.1 | + | 12454 | 0.67 | 0.53202 |
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Target: 5'- cUCAAggcUCUGGAgaaaCAGGGCUUCGaggCGGc -3' miRNA: 3'- cAGUU---AGACCUa---GUCCCGGAGCg--GCU- -5' |
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| 22271 | 5' | -56.1 | NC_005045.1 | + | 36928 | 0.66 | 0.572582 |
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Target: 5'- uGUCccUCgUGGcgCcuguggcuaaugccAGGGCCUCGCUGAa -3' miRNA: 3'- -CAGuuAG-ACCuaG--------------UCCCGGAGCGGCU- -5' |
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| 22271 | 5' | -56.1 | NC_005045.1 | + | 38433 | 0.66 | 0.598171 |
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Target: 5'- aUCGcgCUGGA---GGGCUucaUCGCCGGu -3' miRNA: 3'- cAGUuaGACCUaguCCCGG---AGCGGCU- -5' |
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| 22271 | 5' | -56.1 | NC_005045.1 | + | 28682 | 1.09 | 0.000588 |
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Target: 5'- cGUCAAUCUGGAUCAGGGCCUCGCCGAc -3' miRNA: 3'- -CAGUUAGACCUAGUCCCGGAGCGGCU- -5' |
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| 22271 | 5' | -56.1 | NC_005045.1 | + | 8540 | 0.68 | 0.478987 |
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Target: 5'- -------cGGcgCAGGGCCUCGUCGc -3' miRNA: 3'- caguuagaCCuaGUCCCGGAGCGGCu -5' |
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| 22271 | 5' | -56.1 | NC_005045.1 | + | 31666 | 0.67 | 0.49991 |
|
Target: 5'- cGUCGAgUUGGcccuGUacuuGGGCCUCGUCGAg -3' miRNA: 3'- -CAGUUaGACC----UAgu--CCCGGAGCGGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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