Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 22275 | 3' | -55 | NC_005045.1 | + | 32460 | 0.66 | 0.642005 |
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Target: 5'- gAUACCGGCC-AUCCCGcGAcguGAcacGGCc -3' miRNA: 3'- -UAUGGCCGGuUAGGGUcCUau-CU---CCG- -5' |
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| 22275 | 3' | -55 | NC_005045.1 | + | 33908 | 0.68 | 0.507939 |
|
Target: 5'- -gGCgGGCCAG-CCCAGGGgc--GGCc -3' miRNA: 3'- uaUGgCCGGUUaGGGUCCUaucuCCG- -5' |
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| 22275 | 3' | -55 | NC_005045.1 | + | 34529 | 0.72 | 0.294302 |
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Target: 5'- -cGCCGGagAAUCCCAGcAUGGuGGCg -3' miRNA: 3'- uaUGGCCggUUAGGGUCcUAUCuCCG- -5' |
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| 22275 | 3' | -55 | NC_005045.1 | + | 37055 | 0.68 | 0.518761 |
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Target: 5'- ----aGGCCAGUCCCuGGcc-GAGGUa -3' miRNA: 3'- uauggCCGGUUAGGGuCCuauCUCCG- -5' |
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| 22275 | 3' | -55 | NC_005045.1 | + | 39171 | 0.65 | 0.653355 |
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Target: 5'- -gGCCgGGCCGGUUCCcgacccuGGAcgAGcAGGCg -3' miRNA: 3'- uaUGG-CCGGUUAGGGu------CCUa-UC-UCCG- -5' |
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| 22275 | 3' | -55 | NC_005045.1 | + | 40546 | 0.68 | 0.507939 |
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Target: 5'- -cGCCGGCCuugucgCCCuGGA---AGGCa -3' miRNA: 3'- uaUGGCCGGuua---GGGuCCUaucUCCG- -5' |
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| 22275 | 3' | -55 | NC_005045.1 | + | 42186 | 0.68 | 0.476075 |
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Target: 5'- -aGCCGGaaGcUCCCAGGAggcuAGGCa -3' miRNA: 3'- uaUGGCCggUuAGGGUCCUauc-UCCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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