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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 22290 | 5' | -57.1 | NC_005045.1 | + | 42000 | 0.66 | 0.520413 |
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Target: 5'- gUUgGCUggUGUGGGCGG-GAGUaguuacuccGGGUCc -3' miRNA: 3'- -AAgUGGa-ACAUCCGCCaCUCG---------CCCAG- -5' |
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| 22290 | 5' | -57.1 | NC_005045.1 | + | 25058 | 0.67 | 0.417856 |
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Target: 5'- -gCGCCacuUGGGCGGUGAucaGGGUCu -3' miRNA: 3'- aaGUGGaacAUCCGCCACUcg-CCCAG- -5' |
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| 22290 | 5' | -57.1 | NC_005045.1 | + | 4622 | 0.77 | 0.09557 |
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Target: 5'- cUUCGCCUUGgucacucGGCGGUGAGUucaccuGGGUCc -3' miRNA: 3'- -AAGUGGAACau-----CCGCCACUCG------CCCAG- -5' |
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| 22290 | 5' | -57.1 | NC_005045.1 | + | 41619 | 1.06 | 0.000643 |
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Target: 5'- cUUCACCUUGUAGGCGGUGAGCGGGUCc -3' miRNA: 3'- -AAGUGGAACAUCCGCCACUCGCCCAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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