Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 22297 | 3' | -57 | NC_005045.1 | + | 33911 | 0.66 | 0.49043 |
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Target: 5'- cUCGGCG-GGcCAGCccaGGGGCGGCc- -3' miRNA: 3'- uAGCCGCaUC-GUCGaa-CCCUGUCGug -5' |
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| 22297 | 3' | -57 | NC_005045.1 | + | 32237 | 0.67 | 0.438902 |
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Target: 5'- -cUGGCGUGGguGCUcugcUGGGGucUGGUGCg -3' miRNA: 3'- uaGCCGCAUCguCGA----ACCCU--GUCGUG- -5' |
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| 22297 | 3' | -57 | NC_005045.1 | + | 31154 | 0.67 | 0.399988 |
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Target: 5'- cUgGGCaGUGGCAauGC-UGGGGCAGgCACu -3' miRNA: 3'- uAgCCG-CAUCGU--CGaACCCUGUC-GUG- -5' |
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| 22297 | 3' | -57 | NC_005045.1 | + | 27442 | 0.68 | 0.363349 |
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Target: 5'- uUCGGCGacgUAGUGGCUguccGuGGACGGCGa -3' miRNA: 3'- uAGCCGC---AUCGUCGAa---C-CCUGUCGUg -5' |
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| 22297 | 3' | -57 | NC_005045.1 | + | 37993 | 0.69 | 0.337436 |
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Target: 5'- gAUCGGCGgccagagGGCAGCgccUGGGcGCAGguUc -3' miRNA: 3'- -UAGCCGCa------UCGUCGa--ACCC-UGUCguG- -5' |
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| 22297 | 3' | -57 | NC_005045.1 | + | 23258 | 0.69 | 0.337436 |
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Target: 5'- gGUCGGCGcGGCAGCcgGGGGuauguUGGCAa -3' miRNA: 3'- -UAGCCGCaUCGUCGaaCCCU-----GUCGUg -5' |
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| 22297 | 3' | -57 | NC_005045.1 | + | 9751 | 0.7 | 0.275047 |
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Target: 5'- --aGGCcacGGCAGCcuacgaGGGACAGCACg -3' miRNA: 3'- uagCCGca-UCGUCGaa----CCCUGUCGUG- -5' |
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| 22297 | 3' | -57 | NC_005045.1 | + | 25789 | 0.72 | 0.216278 |
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Target: 5'- -gUGGCGgcGCAGCUUGaGGACcugAGUAUg -3' miRNA: 3'- uaGCCGCauCGUCGAAC-CCUG---UCGUG- -5' |
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| 22297 | 3' | -57 | NC_005045.1 | + | 4399 | 1.08 | 0.000399 |
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Target: 5'- gAUCGGCGUAGCAGCUUGGGACAGCACg -3' miRNA: 3'- -UAGCCGCAUCGUCGAACCCUGUCGUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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