Results 1 - 20 of 27 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
223 | 5' | -58.4 | AC_000008.1 | + | 24345 | 0.66 | 0.387193 |
Target: 5'- -gCGCCAGUGcgccauuAUCUGCGaCGCGUuGCAa -3' miRNA: 3'- aaGCGGUCGU-------UGGAUGC-GCGCGuCGU- -5' |
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223 | 5' | -58.4 | AC_000008.1 | + | 8661 | 0.66 | 0.3789 |
Target: 5'- -gCGCgGGCaggagcugGugCUGCGCGCGUAGg- -3' miRNA: 3'- aaGCGgUCG--------UugGAUGCGCGCGUCgu -5' |
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223 | 5' | -58.4 | AC_000008.1 | + | 18092 | 0.66 | 0.3789 |
Target: 5'- -cCGCCGcGCAugCgacggugcgACGCGCGCcGCc -3' miRNA: 3'- aaGCGGU-CGUugGa--------UGCGCGCGuCGu -5' |
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223 | 5' | -58.4 | AC_000008.1 | + | 27120 | 0.66 | 0.377986 |
Target: 5'- -gCGCCAGC-ACCUGuCGUcaGCGCcauuaugAGCAa -3' miRNA: 3'- aaGCGGUCGuUGGAU-GCG--CGCG-------UCGU- -5' |
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223 | 5' | -58.4 | AC_000008.1 | + | 4519 | 0.66 | 0.369827 |
Target: 5'- gUUGCCcGCGACCauuagAgGUGCGgCGGCAg -3' miRNA: 3'- aAGCGGuCGUUGGa----UgCGCGC-GUCGU- -5' |
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223 | 5' | -58.4 | AC_000008.1 | + | 13436 | 0.66 | 0.360904 |
Target: 5'- -cUGCCAGUGGCgCauaGCGaUGCGCGGCAg -3' miRNA: 3'- aaGCGGUCGUUG-Ga--UGC-GCGCGUCGU- -5' |
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223 | 5' | -58.4 | AC_000008.1 | + | 8277 | 0.66 | 0.358256 |
Target: 5'- -aCGCCGcGCgAGCCcaaaguccagaugucCGCGCGCGGCGg -3' miRNA: 3'- aaGCGGU-CG-UUGGau-------------GCGCGCGUCGU- -5' |
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223 | 5' | -58.4 | AC_000008.1 | + | 19637 | 0.66 | 0.343512 |
Target: 5'- -cCGCCcGuCGGCCggugcgACGUGCGCGGUu -3' miRNA: 3'- aaGCGGuC-GUUGGa-----UGCGCGCGUCGu -5' |
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223 | 5' | -58.4 | AC_000008.1 | + | 22139 | 0.67 | 0.326732 |
Target: 5'- cUCGcCCAGCAGgCgcggGCGgGUGguGCGa -3' miRNA: 3'- aAGC-GGUCGUUgGa---UGCgCGCguCGU- -5' |
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223 | 5' | -58.4 | AC_000008.1 | + | 23674 | 0.67 | 0.310567 |
Target: 5'- -cCGCCGacGUuGCCaGCGCGCGCGGg- -3' miRNA: 3'- aaGCGGU--CGuUGGaUGCGCGCGUCgu -5' |
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223 | 5' | -58.4 | AC_000008.1 | + | 17530 | 0.68 | 0.287473 |
Target: 5'- gUCGCCAGCccGugCUGgccccgauuucCGUGCGCAGgGu -3' miRNA: 3'- aAGCGGUCG--UugGAU-----------GCGCGCGUCgU- -5' |
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223 | 5' | -58.4 | AC_000008.1 | + | 23167 | 0.68 | 0.280081 |
Target: 5'- cUCGCCuuCGAUCUcaGCGCaGCGguGCAg -3' miRNA: 3'- aAGCGGucGUUGGA--UGCG-CGCguCGU- -5' |
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223 | 5' | -58.4 | AC_000008.1 | + | 16181 | 0.68 | 0.277894 |
Target: 5'- gUCGCCAccgccgccgacccgGCAcuGCCgcccaACGCGCgGCGGCGg -3' miRNA: 3'- aAGCGGU--------------CGU--UGGa----UGCGCG-CGUCGU- -5' |
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223 | 5' | -58.4 | AC_000008.1 | + | 12191 | 0.68 | 0.265753 |
Target: 5'- --gGCUGGCGguggcACCcGCGCGCGCuGGCAa -3' miRNA: 3'- aagCGGUCGU-----UGGaUGCGCGCG-UCGU- -5' |
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223 | 5' | -58.4 | AC_000008.1 | + | 31775 | 0.69 | 0.24538 |
Target: 5'- -cCGCUGGUcccgGGCCUAcCGCGCGC-GCAa -3' miRNA: 3'- aaGCGGUCG----UUGGAU-GCGCGCGuCGU- -5' |
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223 | 5' | -58.4 | AC_000008.1 | + | 27226 | 0.69 | 0.238883 |
Target: 5'- ---aCCAGCu-CCUGCccGCGCGCGGCGc -3' miRNA: 3'- aagcGGUCGuuGGAUG--CGCGCGUCGU- -5' |
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223 | 5' | -58.4 | AC_000008.1 | + | 25083 | 0.69 | 0.238883 |
Target: 5'- --gGCCAGUGGCCaaaaaaGCuaGCGCAGCAg -3' miRNA: 3'- aagCGGUCGUUGGa-----UGcgCGCGUCGU- -5' |
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223 | 5' | -58.4 | AC_000008.1 | + | 7017 | 0.69 | 0.225707 |
Target: 5'- cUCGgCGGUGACCUGCacgucuaGgGCGCAGUAg -3' miRNA: 3'- aAGCgGUCGUUGGAUG-------CgCGCGUCGU- -5' |
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223 | 5' | -58.4 | AC_000008.1 | + | 10797 | 0.7 | 0.202874 |
Target: 5'- -gCGCUAGCuuuuuuGGCCacuggcCGCGCGCAGCGu -3' miRNA: 3'- aaGCGGUCG------UUGGau----GCGCGCGUCGU- -5' |
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223 | 5' | -58.4 | AC_000008.1 | + | 24400 | 0.71 | 0.181565 |
Target: 5'- --gGUCGGCGGCCgccacgugUGCGCGCGCGGg- -3' miRNA: 3'- aagCGGUCGUUGG--------AUGCGCGCGUCgu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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