Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 22300 | 3' | -50 | NC_005045.1 | + | 24037 | 0.66 | 0.86525 |
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Target: 5'- cGCAuccgAAGcCGAUaUGGcuGCGCUGCGCGa -3' miRNA: 3'- aCGU----UUU-GCUAgACU--UGCGACGCGUa -5' |
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| 22300 | 3' | -50 | NC_005045.1 | + | 26037 | 0.66 | 0.86525 |
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Target: 5'- aGCAuuGCGAUgCUGGugGC-GCaGCAc -3' miRNA: 3'- aCGUuuUGCUA-GACUugCGaCG-CGUa -5' |
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| 22300 | 3' | -50 | NC_005045.1 | + | 20240 | 0.66 | 0.856394 |
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Target: 5'- aUGCAcgaaguuggGAGCGAUgccgUUG-GCGUUGCGCAUg -3' miRNA: 3'- -ACGU---------UUUGCUA----GACuUGCGACGCGUA- -5' |
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| 22300 | 3' | -50 | NC_005045.1 | + | 23131 | 0.67 | 0.847264 |
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Target: 5'- aGCAAGgucuccugcGCGGcCUGGGC-CUGCGCGg -3' miRNA: 3'- aCGUUU---------UGCUaGACUUGcGACGCGUa -5' |
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| 22300 | 3' | -50 | NC_005045.1 | + | 21360 | 0.67 | 0.818342 |
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Target: 5'- gGCAc--UGAcCUGAGCGgUGCGCAa -3' miRNA: 3'- aCGUuuuGCUaGACUUGCgACGCGUa -5' |
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| 22300 | 3' | -50 | NC_005045.1 | + | 23963 | 0.7 | 0.674091 |
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Target: 5'- gUGCGAaagcAAUGAUUUGcuuCGCUGCGUAUu -3' miRNA: 3'- -ACGUU----UUGCUAGACuu-GCGACGCGUA- -5' |
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| 22300 | 3' | -50 | NC_005045.1 | + | 38203 | 0.7 | 0.662277 |
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Target: 5'- aGCAGAccGCGAgugCUGAGCGgCUGgGCc- -3' miRNA: 3'- aCGUUU--UGCUa--GACUUGC-GACgCGua -5' |
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| 22300 | 3' | -50 | NC_005045.1 | + | 7865 | 0.73 | 0.498862 |
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Target: 5'- uUGCugu-CGGUCUGAACgguacaagGCUGCGCGa -3' miRNA: 3'- -ACGuuuuGCUAGACUUG--------CGACGCGUa -5' |
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| 22300 | 3' | -50 | NC_005045.1 | + | 5361 | 1.07 | 0.002678 |
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Target: 5'- uUGCAAAACGAUCUGAACGCUGCGCAUg -3' miRNA: 3'- -ACGUUUUGCUAGACUUGCGACGCGUA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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