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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 22302 | 5' | -47.2 | NC_005045.1 | + | 17097 | 0.66 | 0.966291 |
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Target: 5'- aUCAGGGuguCGAUcaccuugaacggUCGCGgcggCGGCAUCu -3' miRNA: 3'- -AGUCCU---GCUA------------AGUGCauaaGUCGUAG- -5' |
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| 22302 | 5' | -47.2 | NC_005045.1 | + | 12416 | 0.67 | 0.962444 |
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Target: 5'- --cGGAUGGaUCGCccGUGUUCAGCAc- -3' miRNA: 3'- aguCCUGCUaAGUG--CAUAAGUCGUag -5' |
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| 22302 | 5' | -47.2 | NC_005045.1 | + | 14920 | 0.67 | 0.962444 |
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Target: 5'- -aGGGGCGGcagUCGCGc---CAGCAUCc -3' miRNA: 3'- agUCCUGCUa--AGUGCauaaGUCGUAG- -5' |
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| 22302 | 5' | -47.2 | NC_005045.1 | + | 21637 | 0.67 | 0.958302 |
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Target: 5'- gCGGGACGGca-GCGUGgagCAGCGg- -3' miRNA: 3'- aGUCCUGCUaagUGCAUaa-GUCGUag -5' |
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| 22302 | 5' | -47.2 | NC_005045.1 | + | 28546 | 0.67 | 0.958302 |
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Target: 5'- cUCAGGcCGGg--GCGU--UCGGCGUCa -3' miRNA: 3'- -AGUCCuGCUaagUGCAuaAGUCGUAG- -5' |
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| 22302 | 5' | -47.2 | NC_005045.1 | + | 27902 | 0.69 | 0.899543 |
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Target: 5'- gUCGGGGCGGUgUAUGUggUCGGCu-- -3' miRNA: 3'- -AGUCCUGCUAaGUGCAuaAGUCGuag -5' |
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| 22302 | 5' | -47.2 | NC_005045.1 | + | 3171 | 0.72 | 0.789401 |
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Target: 5'- -uGGGACGGccaUUCGCacagccuuugcgGUAUUCAGCGUUg -3' miRNA: 3'- agUCCUGCU---AAGUG------------CAUAAGUCGUAG- -5' |
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| 22302 | 5' | -47.2 | NC_005045.1 | + | 33222 | 0.72 | 0.789401 |
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Target: 5'- cCAGGACGcggcUCAgggcCGUGU-CAGCAUCg -3' miRNA: 3'- aGUCCUGCua--AGU----GCAUAaGUCGUAG- -5' |
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| 22302 | 5' | -47.2 | NC_005045.1 | + | 30812 | 0.72 | 0.745499 |
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Target: 5'- cCAGGACGGUUC-CGaUGgucUCGGUGUCg -3' miRNA: 3'- aGUCCUGCUAAGuGC-AUa--AGUCGUAG- -5' |
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| 22302 | 5' | -47.2 | NC_005045.1 | + | 6627 | 1.11 | 0.003755 |
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Target: 5'- aUCAGGACGAUUCACGUAUUCAGCAUCc -3' miRNA: 3'- -AGUCCUGCUAAGUGCAUAAGUCGUAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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