Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 22308 | 3' | -62.2 | NC_005045.1 | + | 30846 | 0.67 | 0.269505 |
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Target: 5'- aGCGGCagccuCCucgccgucgaguacGACGAGaacGGUGUgGCCCUGGa -3' miRNA: 3'- -UGCCG-----GG--------------UUGCUC---CCGCAgCGGGACC- -5' |
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| 22308 | 3' | -62.2 | NC_005045.1 | + | 28806 | 0.67 | 0.2715 |
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Target: 5'- cCGGCcaCCAGCGGGGacCGUUGCCCcgccgUGGu -3' miRNA: 3'- uGCCG--GGUUGCUCCc-GCAGCGGG-----ACC- -5' |
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| 22308 | 3' | -62.2 | NC_005045.1 | + | 28590 | 0.67 | 0.292103 |
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Target: 5'- gUGGCCCuggauaucgAGCGGGaggucGGCGagGCCCUGa -3' miRNA: 3'- uGCCGGG---------UUGCUC-----CCGCagCGGGACc -5' |
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| 22308 | 3' | -62.2 | NC_005045.1 | + | 31309 | 0.67 | 0.299238 |
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Target: 5'- uAUGGCCUuccagaugGGCGaAGGGCGU-GCCC-GGc -3' miRNA: 3'- -UGCCGGG--------UUGC-UCCCGCAgCGGGaCC- -5' |
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| 22308 | 3' | -62.2 | NC_005045.1 | + | 35944 | 0.66 | 0.321446 |
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Target: 5'- gACGcCgCCAAUGAGGcgcuGCGccgUGCCCUGGg -3' miRNA: 3'- -UGCcG-GGUUGCUCC----CGCa--GCGGGACC- -5' |
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| 22308 | 3' | -62.2 | NC_005045.1 | + | 39641 | 0.66 | 0.329118 |
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Target: 5'- gACGGCggCGACGAGcuGuCGUaUGCCCUGGg -3' miRNA: 3'- -UGCCGg-GUUGCUCc-C-GCA-GCGGGACC- -5' |
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| 22308 | 3' | -62.2 | NC_005045.1 | + | 40595 | 0.66 | 0.329118 |
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Target: 5'- -aGG-CCGGCGAGGGCaGcCGCCa-GGg -3' miRNA: 3'- ugCCgGGUUGCUCCCG-CaGCGGgaCC- -5' |
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| 22308 | 3' | -62.2 | NC_005045.1 | + | 18692 | 0.66 | 0.344865 |
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Target: 5'- gGCGGCCCuggccggagcguGAUGcuGGCGccgccgCGaCCCUGGa -3' miRNA: 3'- -UGCCGGG------------UUGCucCCGCa-----GC-GGGACC- -5' |
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| 22308 | 3' | -62.2 | NC_005045.1 | + | 25496 | 0.66 | 0.344865 |
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Target: 5'- uGCGGCCCGugGGGucaGGUGUCaGCUUg-- -3' miRNA: 3'- -UGCCGGGUugCUC---CCGCAG-CGGGacc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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