Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 22308 | 3' | -62.2 | NC_005045.1 | + | 33360 | 0.74 | 0.084985 |
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Target: 5'- cUGGCCCAGCccggaGAGGGCGcacggCGCCUccUGGg -3' miRNA: 3'- uGCCGGGUUG-----CUCCCGCa----GCGGG--ACC- -5' |
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| 22308 | 3' | -62.2 | NC_005045.1 | + | 33444 | 0.72 | 0.131535 |
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Target: 5'- gACGGCCCAG-GAGGcGcCGUgCGCCCUcuccGGg -3' miRNA: 3'- -UGCCGGGUUgCUCC-C-GCA-GCGGGA----CC- -5' |
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| 22308 | 3' | -62.2 | NC_005045.1 | + | 33527 | 0.7 | 0.176183 |
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Target: 5'- gAUGGCUgAGC-AGGGCGUUGgCCUGa -3' miRNA: 3'- -UGCCGGgUUGcUCCCGCAGCgGGACc -5' |
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| 22308 | 3' | -62.2 | NC_005045.1 | + | 35944 | 0.66 | 0.321446 |
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Target: 5'- gACGcCgCCAAUGAGGcgcuGCGccgUGCCCUGGg -3' miRNA: 3'- -UGCcG-GGUUGCUCC----CGCa--GCGGGACC- -5' |
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| 22308 | 3' | -62.2 | NC_005045.1 | + | 37997 | 0.69 | 0.211215 |
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Target: 5'- gGCGGCCa---GAGGGCa--GCgCCUGGg -3' miRNA: 3'- -UGCCGGguugCUCCCGcagCG-GGACC- -5' |
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| 22308 | 3' | -62.2 | NC_005045.1 | + | 39641 | 0.66 | 0.329118 |
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Target: 5'- gACGGCggCGACGAGcuGuCGUaUGCCCUGGg -3' miRNA: 3'- -UGCCGg-GUUGCUCc-C-GCA-GCGGGACC- -5' |
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| 22308 | 3' | -62.2 | NC_005045.1 | + | 40534 | 0.69 | 0.190517 |
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Target: 5'- gGCGGCugCCcuCGccGGCcuuGUCGCCCUGGa -3' miRNA: 3'- -UGCCG--GGuuGCucCCG---CAGCGGGACC- -5' |
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| 22308 | 3' | -62.2 | NC_005045.1 | + | 40595 | 0.66 | 0.329118 |
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Target: 5'- -aGG-CCGGCGAGGGCaGcCGCCa-GGg -3' miRNA: 3'- ugCCgGGUUGCUCCCG-CaGCGGgaCC- -5' |
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| 22308 | 3' | -62.2 | NC_005045.1 | + | 41205 | 0.69 | 0.216131 |
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Target: 5'- cACGGUCCAccuccugGCGcuGGGUGUUGgUCCUGGc -3' miRNA: 3'- -UGCCGGGU-------UGCu-CCCGCAGC-GGGACC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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