Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 22308 | 5' | -57.6 | NC_005045.1 | + | 41577 | 0.66 | 0.498895 |
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Target: 5'- -gCCCUCGCcGCC-CAggGCAgUGGCuGCu -3' miRNA: 3'- aaGGGAGCGcUGGaGUa-CGU-ACCG-CG- -5' |
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| 22308 | 5' | -57.6 | NC_005045.1 | + | 3641 | 0.66 | 0.478065 |
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Target: 5'- -gCCUUCGuCGAacuaugguuCCUCGgcgGCGUGGuCGCu -3' miRNA: 3'- aaGGGAGC-GCU---------GGAGUa--CGUACC-GCG- -5' |
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| 22308 | 5' | -57.6 | NC_005045.1 | + | 37317 | 0.66 | 0.467812 |
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Target: 5'- ---aCUCGCGGCagUC-UGuCGUGGCGCg -3' miRNA: 3'- aaggGAGCGCUGg-AGuAC-GUACCGCG- -5' |
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| 22308 | 5' | -57.6 | NC_005045.1 | + | 9519 | 0.66 | 0.457673 |
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Target: 5'- -gCCCUCGUugGGCC--GUGCAUcagaguacaGGCGCa -3' miRNA: 3'- aaGGGAGCG--CUGGagUACGUA---------CCGCG- -5' |
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| 22308 | 5' | -57.6 | NC_005045.1 | + | 39943 | 0.68 | 0.381143 |
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Target: 5'- -aCCCU-GCGGCCcaUCAUGCA--GCGCc -3' miRNA: 3'- aaGGGAgCGCUGG--AGUACGUacCGCG- -5' |
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| 22308 | 5' | -57.6 | NC_005045.1 | + | 39539 | 0.68 | 0.363388 |
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Target: 5'- -gCCaCUUGCucagGACacuCUCGUGCGUGGCGUc -3' miRNA: 3'- aaGG-GAGCG----CUG---GAGUACGUACCGCG- -5' |
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| 22308 | 5' | -57.6 | NC_005045.1 | + | 9687 | 0.68 | 0.354729 |
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Target: 5'- gUCCCUCGUaggcugccguGGCCUgGaggGCAUGGaCGUg -3' miRNA: 3'- aAGGGAGCG----------CUGGAgUa--CGUACC-GCG- -5' |
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| 22308 | 5' | -57.6 | NC_005045.1 | + | 41176 | 0.75 | 0.118464 |
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Target: 5'- gUCCUggcaGCGACCUCcuUGaCGUGGCGCu -3' miRNA: 3'- aAGGGag--CGCUGGAGu-AC-GUACCGCG- -5' |
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| 22308 | 5' | -57.6 | NC_005045.1 | + | 9495 | 1.08 | 0.000417 |
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Target: 5'- gUUCCCUCGCGACCUCAUGCAUGGCGCc -3' miRNA: 3'- -AAGGGAGCGCUGGAGUACGUACCGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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