Results 21 - 25 of 25 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 22314 | 3' | -55 | NC_005045.1 | + | 20155 | 0.69 | 0.51264 |
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Target: 5'- aCCCCaugCGCAACgccaacgGCAUCGcucccaacuucgugCAUGGCCu -3' miRNA: 3'- -GGGG---GCGUUGa------UGUAGCa-------------GUACCGGc -5' |
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| 22314 | 3' | -55 | NC_005045.1 | + | 29942 | 0.69 | 0.497887 |
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Target: 5'- gCCCUGCAuGCguuggGCAUUgcccaggccgGUCAUGGCCu -3' miRNA: 3'- gGGGGCGU-UGa----UGUAG----------CAGUACCGGc -5' |
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| 22314 | 3' | -55 | NC_005045.1 | + | 39107 | 0.72 | 0.366473 |
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Target: 5'- aCUCCUGCGACUGCcgUGUCAgcuucgcccgcucggUGGCgGu -3' miRNA: 3'- -GGGGGCGUUGAUGuaGCAGU---------------ACCGgC- -5' |
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| 22314 | 3' | -55 | NC_005045.1 | + | 35435 | 0.73 | 0.28336 |
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Target: 5'- aCCCCGCAGCcgccaccggcgAUAUCGUCAgcgGcGCCGc -3' miRNA: 3'- gGGGGCGUUGa----------UGUAGCAGUa--C-CGGC- -5' |
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| 22314 | 3' | -55 | NC_005045.1 | + | 11316 | 1.12 | 0.000528 |
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Target: 5'- aCCCCCGCAACUACAUCGUCAUGGCCGg -3' miRNA: 3'- -GGGGGCGUUGAUGUAGCAGUACCGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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