Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 22358 | 3' | -57.7 | NC_005045.1 | + | 34510 | 0.67 | 0.463536 |
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Target: 5'- -gGUGgCGCUGGUCagGCCGC-UGGCc -3' miRNA: 3'- cgCGCgGUGACUAGgaUGGCGcACCG- -5' |
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| 22358 | 3' | -57.7 | NC_005045.1 | + | 31052 | 0.67 | 0.434145 |
|
Target: 5'- cCGUGCCGCUGA-CCUugaCGU-UGGCa -3' miRNA: 3'- cGCGCGGUGACUaGGAug-GCGcACCG- -5' |
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| 22358 | 3' | -57.7 | NC_005045.1 | + | 27333 | 0.7 | 0.328338 |
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Target: 5'- -gGUGCCGCccugguaucgaGGUCgUACUGCGUGGUg -3' miRNA: 3'- cgCGCGGUGa----------CUAGgAUGGCGCACCG- -5' |
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| 22358 | 3' | -57.7 | NC_005045.1 | + | 9071 | 0.72 | 0.236625 |
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Target: 5'- gGUGCGgUuCUGGUCCUugCGC-UGGCc -3' miRNA: 3'- -CGCGCgGuGACUAGGAugGCGcACCG- -5' |
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| 22358 | 3' | -57.7 | NC_005045.1 | + | 37385 | 0.72 | 0.213073 |
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Target: 5'- aGCGCGCCACgacagaCUGCCGCGaGuGCc -3' miRNA: 3'- -CGCGCGGUGacuag-GAUGGCGCaC-CG- -5' |
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| 22358 | 3' | -57.7 | NC_005045.1 | + | 35517 | 0.73 | 0.186493 |
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Target: 5'- uGCgGCGCCGCUGAcgaUAUCGCcgGUGGCg -3' miRNA: 3'- -CG-CGCGGUGACUaggAUGGCG--CACCG- -5' |
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| 22358 | 3' | -57.7 | NC_005045.1 | + | 14909 | 0.66 | 0.535808 |
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Target: 5'- uCGCGCCAgc-AUCC-GCCGCGacguugGGCa -3' miRNA: 3'- cGCGCGGUgacUAGGaUGGCGCa-----CCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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