Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 22358 | 3' | -57.7 | NC_005045.1 | + | 34510 | 0.67 | 0.463536 |
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Target: 5'- -gGUGgCGCUGGUCagGCCGC-UGGCc -3' miRNA: 3'- cgCGCgGUGACUAGgaUGGCGcACCG- -5' |
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| 22358 | 3' | -57.7 | NC_005045.1 | + | 26416 | 0.67 | 0.479631 |
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Target: 5'- -gGCGUCACguaagugGCCGUGUGGCu -3' miRNA: 3'- cgCGCGGUGacuaggaUGGCGCACCG- -5' |
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| 22358 | 3' | -57.7 | NC_005045.1 | + | 42108 | 0.66 | 0.508431 |
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Target: 5'- gGUGgGCCACUGccuagccuccugggaGcuUCCggcuucGCCGgGUGGCg -3' miRNA: 3'- -CGCgCGGUGAC---------------U--AGGa-----UGGCgCACCG- -5' |
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| 22358 | 3' | -57.7 | NC_005045.1 | + | 45 | 0.66 | 0.508431 |
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Target: 5'- gGUGgGCCACUGccuagccuccugggaGcuUCCggcuucGCCGgGUGGCg -3' miRNA: 3'- -CGCgCGGUGAC---------------U--AGGa-----UGGCgCACCG- -5' |
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| 22358 | 3' | -57.7 | NC_005045.1 | + | 7754 | 0.66 | 0.514699 |
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Target: 5'- aGCGCGCCca--AUCCagcCCGCGUcGGUa -3' miRNA: 3'- -CGCGCGGugacUAGGau-GGCGCA-CCG- -5' |
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| 22358 | 3' | -57.7 | NC_005045.1 | + | 739 | 0.66 | 0.532621 |
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Target: 5'- -aGUGCCACgcgGGuuucagggugguggUCCUgGCUGgGUGGCu -3' miRNA: 3'- cgCGCGGUGa--CU--------------AGGA-UGGCgCACCG- -5' |
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| 22358 | 3' | -57.7 | NC_005045.1 | + | 14909 | 0.66 | 0.535808 |
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Target: 5'- uCGCGCCAgc-AUCC-GCCGCGacguugGGCa -3' miRNA: 3'- cGCGCGGUgacUAGGaUGGCGCa-----CCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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