Results 1 - 11 of 11 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22362 | 5' | -57.2 | NC_005045.1 | + | 32779 | 1.08 | 0.000541 |
Target: 5'- aCCAACCCGGUGACCGUGCUCCACGAAg -3' miRNA: 3'- -GGUUGGGCCACUGGCACGAGGUGCUU- -5' |
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22362 | 5' | -57.2 | NC_005045.1 | + | 31744 | 0.72 | 0.237714 |
Target: 5'- gCCGGCCCu-UGACCGUGCUuCCGCa-- -3' miRNA: 3'- -GGUUGGGccACUGGCACGA-GGUGcuu -5' |
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22362 | 5' | -57.2 | NC_005045.1 | + | 41140 | 0.7 | 0.322414 |
Target: 5'- cCCAgcGCCaggaGGUgGACCGUGC-CCugGAc -3' miRNA: 3'- -GGU--UGGg---CCA-CUGGCACGaGGugCUu -5' |
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22362 | 5' | -57.2 | NC_005045.1 | + | 18002 | 0.69 | 0.34683 |
Target: 5'- uUCGGCCagGGUGACCGccUGUUCCAgGGu -3' miRNA: 3'- -GGUUGGg-CCACUGGC--ACGAGGUgCUu -5' |
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22362 | 5' | -57.2 | NC_005045.1 | + | 23359 | 0.69 | 0.355258 |
Target: 5'- aCCAG-CCGGUGGCgGUGCcugCCGCa-- -3' miRNA: 3'- -GGUUgGGCCACUGgCACGa--GGUGcuu -5' |
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22362 | 5' | -57.2 | NC_005045.1 | + | 33240 | 0.69 | 0.381396 |
Target: 5'- gCCAACaagggcgcuCCGGUGACCGaggagGCUagCCugGAGa -3' miRNA: 3'- -GGUUG---------GGCCACUGGCa----CGA--GGugCUU- -5' |
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22362 | 5' | -57.2 | NC_005045.1 | + | 28417 | 0.69 | 0.390389 |
Target: 5'- aCAACCUGGgcGCCGcUGC-CCGCGAu -3' miRNA: 3'- gGUUGGGCCacUGGC-ACGaGGUGCUu -5' |
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22362 | 5' | -57.2 | NC_005045.1 | + | 32065 | 0.68 | 0.416294 |
Target: 5'- gCAGCCCuggcaguugaugcGGUuacuuucggcgcuGGCCGUGCUCUGCGGc -3' miRNA: 3'- gGUUGGG-------------CCA-------------CUGGCACGAGGUGCUu -5' |
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22362 | 5' | -57.2 | NC_005045.1 | + | 12245 | 0.67 | 0.467068 |
Target: 5'- gCCAGCUCGGUGAUgGUGggCCAg--- -3' miRNA: 3'- -GGUUGGGCCACUGgCACgaGGUgcuu -5' |
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22362 | 5' | -57.2 | NC_005045.1 | + | 33961 | 0.66 | 0.518738 |
Target: 5'- gUCAGCCCGGUccaGGCCaggGaugCCACGGAa -3' miRNA: 3'- -GGUUGGGCCA---CUGGca-Cga-GGUGCUU- -5' |
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22362 | 5' | -57.2 | NC_005045.1 | + | 39854 | 0.66 | 0.550806 |
Target: 5'- aUCGACCCGGauACCGgcaaGCUCCguuAUGAGg -3' miRNA: 3'- -GGUUGGGCCacUGGCa---CGAGG---UGCUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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