Results 1 - 8 of 8 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22363 | 5' | -57.3 | NC_005045.1 | + | 21528 | 0.66 | 0.516021 |
Target: 5'- aCUCGACGAcGCcGGaaUCAgGCGGGcacaGGCg -3' miRNA: 3'- -GAGUUGCU-CGuCC--AGUgCGCCC----UCGa -5' |
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22363 | 5' | -57.3 | NC_005045.1 | + | 19473 | 0.66 | 0.53739 |
Target: 5'- gCUCuGCGccaucGCAGGUC-UGCuGGAGCUg -3' miRNA: 3'- -GAGuUGCu----CGUCCAGuGCGcCCUCGA- -5' |
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22363 | 5' | -57.3 | NC_005045.1 | + | 29027 | 0.67 | 0.454199 |
Target: 5'- -aCAACGugcAGCAGGaaguUCuGCGCGGuGAGCg -3' miRNA: 3'- gaGUUGC---UCGUCC----AG-UGCGCC-CUCGa -5' |
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22363 | 5' | -57.3 | NC_005045.1 | + | 7788 | 0.67 | 0.484639 |
Target: 5'- aUCAAUGGGCAGGcCA--UGGGuGCUg -3' miRNA: 3'- gAGUUGCUCGUCCaGUgcGCCCuCGA- -5' |
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22363 | 5' | -57.3 | NC_005045.1 | + | 7292 | 0.67 | 0.444284 |
Target: 5'- cCUgGAgGuGcCAGGUCAUGCGGGcGCc -3' miRNA: 3'- -GAgUUgCuC-GUCCAGUGCGCCCuCGa -5' |
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22363 | 5' | -57.3 | NC_005045.1 | + | 32846 | 0.69 | 0.327546 |
Target: 5'- uUCGugGAGCAcGGUCAC-CGGGuuGGUg -3' miRNA: 3'- gAGUugCUCGU-CCAGUGcGCCC--UCGa -5' |
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22363 | 5' | -57.3 | NC_005045.1 | + | 39508 | 0.72 | 0.228996 |
Target: 5'- gUCAAUGAacaGCAGGUCGCGCaGcuuGAGCUg -3' miRNA: 3'- gAGUUGCU---CGUCCAGUGCGcC---CUCGA- -5' |
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22363 | 5' | -57.3 | NC_005045.1 | + | 33599 | 1.08 | 0.000518 |
Target: 5'- gCUCAACGAGCAGGUCACGCGGGAGCUg -3' miRNA: 3'- -GAGUUGCUCGUCCAGUGCGCCCUCGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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