Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 22369 | 3' | -49.5 | NC_005045.1 | + | 31674 | 0.68 | 0.811099 |
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Target: 5'- --aGGCACC-GCGUCgAGUUGGCCc- -3' miRNA: 3'- cugUUGUGGuUGUAGgUUAACCGGca -5' |
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| 22369 | 3' | -49.5 | NC_005045.1 | + | 26101 | 0.69 | 0.779886 |
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Target: 5'- uGCGcCACCAGCAUCgCAAUgcugcGGCCa- -3' miRNA: 3'- cUGUuGUGGUUGUAG-GUUAa----CCGGca -5' |
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| 22369 | 3' | -49.5 | NC_005045.1 | + | 13836 | 0.7 | 0.724531 |
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Target: 5'- gGACAGCGCCAug--CCAAU-GGCCuGUa -3' miRNA: 3'- -CUGUUGUGGUuguaGGUUAaCCGG-CA- -5' |
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| 22369 | 3' | -49.5 | NC_005045.1 | + | 37753 | 0.71 | 0.678257 |
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Target: 5'- uGGCGACGCCAcCGUCCAcgUagucgcugcGGUCGUu -3' miRNA: 3'- -CUGUUGUGGUuGUAGGUuaA---------CCGGCA- -5' |
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| 22369 | 3' | -49.5 | NC_005045.1 | + | 14915 | 0.71 | 0.677086 |
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Target: 5'- cGGCAgucGCGCCAGCAUCCGccgcgacGUUGGgCa- -3' miRNA: 3'- -CUGU---UGUGGUUGUAGGU-------UAACCgGca -5' |
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| 22369 | 3' | -49.5 | NC_005045.1 | + | 5053 | 0.71 | 0.635894 |
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Target: 5'- gGACGGCGCgGauggcgcuggccuucACGUCCAGcagcUUGGCCGg -3' miRNA: 3'- -CUGUUGUGgU---------------UGUAGGUU----AACCGGCa -5' |
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| 22369 | 3' | -49.5 | NC_005045.1 | + | 18768 | 0.75 | 0.418523 |
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Target: 5'- cGGCGGCGCCAGCAucacgcUCCGGccagGGCCGc -3' miRNA: 3'- -CUGUUGUGGUUGU------AGGUUaa--CCGGCa -5' |
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| 22369 | 3' | -49.5 | NC_005045.1 | + | 10268 | 0.68 | 0.840372 |
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Target: 5'- -cCAGCACCAGaccggaGUCCA---GGCCGg -3' miRNA: 3'- cuGUUGUGGUUg-----UAGGUuaaCCGGCa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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