Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 22369 | 3' | -49.5 | NC_005045.1 | + | 10268 | 0.68 | 0.840372 |
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Target: 5'- -cCAGCACCAGaccggaGUCCA---GGCCGg -3' miRNA: 3'- cuGUUGUGGUUg-----UAGGUuaaCCGGCa -5' |
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| 22369 | 3' | -49.5 | NC_005045.1 | + | 32987 | 0.67 | 0.849641 |
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Target: 5'- cGGCAACuCCAACA-CC--UUGGCCa- -3' miRNA: 3'- -CUGUUGuGGUUGUaGGuuAACCGGca -5' |
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| 22369 | 3' | -49.5 | NC_005045.1 | + | 13589 | 0.67 | 0.875837 |
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Target: 5'- cGACAuCGCCGACGUuCCGuucccggcagUGGCUGa -3' miRNA: 3'- -CUGUuGUGGUUGUA-GGUua--------ACCGGCa -5' |
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| 22369 | 3' | -49.5 | NC_005045.1 | + | 36151 | 0.66 | 0.899433 |
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Target: 5'- cGCGACcUCAACAUCg---UGGCCGg -3' miRNA: 3'- cUGUUGuGGUUGUAGguuaACCGGCa -5' |
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| 22369 | 3' | -49.5 | NC_005045.1 | + | 41364 | 0.66 | 0.905977 |
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Target: 5'- uGGCgAACACCcGCGagCAGUaccucgcUGGCCGUa -3' miRNA: 3'- -CUG-UUGUGGuUGUagGUUA-------ACCGGCA- -5' |
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| 22369 | 3' | -49.5 | NC_005045.1 | + | 41134 | 0.66 | 0.906689 |
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Target: 5'- -cCAACAcCCAGCG-CCAGgaggUGGaCCGUg -3' miRNA: 3'- cuGUUGU-GGUUGUaGGUUa---ACC-GGCA- -5' |
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| 22369 | 3' | -49.5 | NC_005045.1 | + | 19816 | 0.66 | 0.906689 |
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Target: 5'- uACGGCACC-ACGUUCAAggGcGUCGUg -3' miRNA: 3'- cUGUUGUGGuUGUAGGUUaaC-CGGCA- -5' |
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| 22369 | 3' | -49.5 | NC_005045.1 | + | 22059 | 0.66 | 0.913633 |
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Target: 5'- cGCGguGCGCCGAgAUgCGAcUGGCCGc -3' miRNA: 3'- cUGU--UGUGGUUgUAgGUUaACCGGCa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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