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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 22370 | 3' | -51.4 | NC_005045.1 | + | 32853 | 0.71 | 0.512525 |
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Target: 5'- gGACCGCUUcGUGGAGCACgguCACCggGUu -3' miRNA: 3'- gUUGGCGAA-CGUCUUGUG---GUGGuaCG- -5' |
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| 22370 | 3' | -51.4 | NC_005045.1 | + | 14103 | 0.71 | 0.523636 |
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Target: 5'- gGGCgGCUUGCAGAGCGuuGaguuguugagUCAUGCg -3' miRNA: 3'- gUUGgCGAACGUCUUGUggU----------GGUACG- -5' |
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| 22370 | 3' | -51.4 | NC_005045.1 | + | 40583 | 0.71 | 0.534839 |
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Target: 5'- gCAGCCGC---CAGGGCugCGCCGcGCa -3' miRNA: 3'- -GUUGGCGaacGUCUUGugGUGGUaCG- -5' |
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| 22370 | 3' | -51.4 | NC_005045.1 | + | 22107 | 0.7 | 0.603498 |
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Target: 5'- -cACCGCguaggaGCGGAGCG--ACCAUGCg -3' miRNA: 3'- guUGGCGaa----CGUCUUGUggUGGUACG- -5' |
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| 22370 | 3' | -51.4 | NC_005045.1 | + | 39541 | 0.69 | 0.638329 |
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Target: 5'- aGGCCaCUUGCucAGGACACUcUCGUGCg -3' miRNA: 3'- gUUGGcGAACG--UCUUGUGGuGGUACG- -5' |
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| 22370 | 3' | -51.4 | NC_005045.1 | + | 32205 | 0.69 | 0.649942 |
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Target: 5'- --cCCGaCUUGCccAGCGCCGCCA-GCa -3' miRNA: 3'- guuGGC-GAACGucUUGUGGUGGUaCG- -5' |
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| 22370 | 3' | -51.4 | NC_005045.1 | + | 32727 | 0.69 | 0.661537 |
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Target: 5'- aGGCagaGCUgGCAGuguCGCCGCCcUGCa -3' miRNA: 3'- gUUGg--CGAaCGUCuu-GUGGUGGuACG- -5' |
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| 22370 | 3' | -51.4 | NC_005045.1 | + | 7983 | 0.69 | 0.669636 |
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Target: 5'- aGGCgCGCUUGCAGGucugcugcaacaugAuCGCCGCCA-GCc -3' miRNA: 3'- gUUG-GCGAACGUCU--------------U-GUGGUGGUaCG- -5' |
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| 22370 | 3' | -51.4 | NC_005045.1 | + | 17870 | 0.69 | 0.684622 |
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Target: 5'- gCGGCgGCcaUUGCAuuGAACGCC-UCAUGCa -3' miRNA: 3'- -GUUGgCG--AACGU--CUUGUGGuGGUACG- -5' |
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| 22370 | 3' | -51.4 | NC_005045.1 | + | 10183 | 0.84 | 0.093557 |
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Target: 5'- --cCCGC-UGCGGGACACCacGCCAUGCg -3' miRNA: 3'- guuGGCGaACGUCUUGUGG--UGGUACG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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