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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 22370 | 3' | -51.4 | NC_005045.1 | + | 33259 | 0.66 | 0.824465 |
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Target: 5'- uGACCGaggagGCuagccuGGAgACCAUCGUGCa -3' miRNA: 3'- gUUGGCgaa--CGu-----CUUgUGGUGGUACG- -5' |
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| 22370 | 3' | -51.4 | NC_005045.1 | + | 34446 | 0.73 | 0.427693 |
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Target: 5'- cCAGCgGCcUGaccAGCGCCACCAUGCu -3' miRNA: 3'- -GUUGgCGaACgucUUGUGGUGGUACG- -5' |
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| 22370 | 3' | -51.4 | NC_005045.1 | + | 35946 | 0.66 | 0.843293 |
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Target: 5'- -cGCCGCcaaUGaGGcGCugCGCCGUGCc -3' miRNA: 3'- guUGGCGa--ACgUCuUGugGUGGUACG- -5' |
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| 22370 | 3' | -51.4 | NC_005045.1 | + | 36188 | 1.13 | 0.000887 |
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Target: 5'- uCAACCGCUUGCAGAACACCACCAUGCa -3' miRNA: 3'- -GUUGGCGAACGUCUUGUGGUGGUACG- -5' |
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| 22370 | 3' | -51.4 | NC_005045.1 | + | 36480 | 0.66 | 0.843293 |
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Target: 5'- ---aUGCUugUGCGGGACGCUACCcUGa -3' miRNA: 3'- guugGCGA--ACGUCUUGUGGUGGuACg -5' |
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| 22370 | 3' | -51.4 | NC_005045.1 | + | 36976 | 0.75 | 0.317518 |
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Target: 5'- gCAACCGCgcagUGUGGGAUuCCACCAcugGCg -3' miRNA: 3'- -GUUGGCGa---ACGUCUUGuGGUGGUa--CG- -5' |
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| 22370 | 3' | -51.4 | NC_005045.1 | + | 38206 | 0.66 | 0.814707 |
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Target: 5'- aGACCGCgagUGCuGAGCGgCUggGCC-UGCu -3' miRNA: 3'- gUUGGCGa--ACGuCUUGU-GG--UGGuACG- -5' |
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| 22370 | 3' | -51.4 | NC_005045.1 | + | 39541 | 0.69 | 0.638329 |
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Target: 5'- aGGCCaCUUGCucAGGACACUcUCGUGCg -3' miRNA: 3'- gUUGGcGAACG--UCUUGUGGuGGUACG- -5' |
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| 22370 | 3' | -51.4 | NC_005045.1 | + | 39889 | 0.66 | 0.843293 |
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Target: 5'- gCAugUGgUUGCGGAagagcugaccaACugCACCA-GCg -3' miRNA: 3'- -GUugGCgAACGUCU-----------UGugGUGGUaCG- -5' |
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| 22370 | 3' | -51.4 | NC_005045.1 | + | 40583 | 0.71 | 0.534839 |
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Target: 5'- gCAGCCGC---CAGGGCugCGCCGcGCa -3' miRNA: 3'- -GUUGGCGaacGUCUUGugGUGGUaCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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