Results 41 - 44 of 44 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22372 | 5' | -51.3 | NC_005045.1 | + | 24037 | 0.66 | 0.837961 |
Target: 5'- gGACAG-AGCuGCCAGG-GCAGguGCCGCa -3' miRNA: 3'- -CUGUUaUUGcCGGUCUgUGUC--UGGCG- -5' |
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22372 | 5' | -51.3 | NC_005045.1 | + | 18678 | 0.66 | 0.855101 |
Target: 5'- cGGCAAUGaccugggGCGGCCcuGGC-CGGAgCGUg -3' miRNA: 3'- -CUGUUAU-------UGCCGGu-CUGuGUCUgGCG- -5' |
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22372 | 5' | -51.3 | NC_005045.1 | + | 16218 | 0.66 | 0.837961 |
Target: 5'- cACGAauGCGGCgGGuucCugGGGCUGCa -3' miRNA: 3'- cUGUUauUGCCGgUCu--GugUCUGGCG- -5' |
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22372 | 5' | -51.3 | NC_005045.1 | + | 8791 | 0.66 | 0.863754 |
Target: 5'- cACAggAugGGCCGGcCugGGucgaaguACCGCu -3' miRNA: 3'- cUGUuaUugCCGGUCuGugUC-------UGGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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