miRNA: 3'- -CUGUUAUUGCCG--G--------------UCUGUG------UCUGGCG- -5'
miRNA: 3'- -CUGUUa-----UUGCCGGU--CUG---------UGUCUGGCG- -5'
miRNA: 3'- cUGUuaUUG-CCGG----UCUGuGUCUGGCG- -5'
miRNA: 3'- -CUGUUAUUGCCGGUCUGugUCUGGCG- -5'
Results 41 - 44 of 44 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
|
Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 22372 | 5' | -51.3 | NC_005045.1 | + | 14120 | 0.68 | 0.754054 |
|
Target: 5'- cGGCGGUGGCGGCggUcgcaucuucccggcGGGCACcgccauGGGCCGCu -3' miRNA: 3'- -CUGUUAUUGCCG--G--------------UCUGUG------UCUGGCG- -5' |
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| 22372 | 5' | -51.3 | NC_005045.1 | + | 29981 | 0.68 | 0.746444 |
|
Target: 5'- gGACAAgaugcaGGCGGCCAacGGCucaagccugACGGAUCGCc -3' miRNA: 3'- -CUGUUa-----UUGCCGGU--CUG---------UGUCUGGCG- -5' |
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| 22372 | 5' | -51.3 | NC_005045.1 | + | 23044 | 0.68 | 0.746444 |
|
Target: 5'- aGCGucAGCaGGCCgcgcAGGCcCAGGCCGCg -3' miRNA: 3'- cUGUuaUUG-CCGG----UCUGuGUCUGGCG- -5' |
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| 22372 | 5' | -51.3 | NC_005045.1 | + | 37355 | 0.95 | 0.017904 |
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Target: 5'- gGACAAUAACGGCCAGACcaAGACCGCc -3' miRNA: 3'- -CUGUUAUUGCCGGUCUGugUCUGGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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