miRNA: 3'- cUGUUauUGCc--GGUCUGUGUCUGGCG- -5'
miRNA: 3'- cUGUUauuGCCGGUCUGUGU----------CUGGCG- -5'
miRNA: 3'- -CU-GUUAUUGCCGG--UCugUGUCU--GGCG- -5'
miRNA: 3'- -CUGUUAUUGCCGGUCUGugUCUGGCG- -5'
Results 41 - 44 of 44 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 22372 | 5' | -51.3 | NC_005045.1 | + | 9898 | 0.75 | 0.367182 |
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Target: 5'- uGCAGguACGcacCCAGGCGCGGGCCGCc -3' miRNA: 3'- cUGUUauUGCc--GGUCUGUGUCUGGCG- -5' |
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| 22372 | 5' | -51.3 | NC_005045.1 | + | 12560 | 0.76 | 0.332089 |
|
Target: 5'- uGCAGcu-CGGCCAGGCGCAucaacagcuuGGCCGCc -3' miRNA: 3'- cUGUUauuGCCGGUCUGUGU----------CUGGCG- -5' |
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| 22372 | 5' | -51.3 | NC_005045.1 | + | 23898 | 0.78 | 0.235406 |
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Target: 5'- cGAcCGAUGGCGGCCuuGGugGCAGGguCCGCg -3' miRNA: 3'- -CU-GUUAUUGCCGG--UCugUGUCU--GGCG- -5' |
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| 22372 | 5' | -51.3 | NC_005045.1 | + | 37355 | 0.95 | 0.017904 |
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Target: 5'- gGACAAUAACGGCCAGACcaAGACCGCc -3' miRNA: 3'- -CUGUUAUUGCCGGUCUGugUCUGGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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