Results 1 - 20 of 22 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22375 | 3' | -57.3 | NC_005045.1 | + | 39641 | 1.1 | 0.000391 |
Target: 5'- gACGGCGGCGACGAGCUGUCGUAUGCCc -3' miRNA: 3'- -UGCCGCCGCUGCUCGACAGCAUACGG- -5' |
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22375 | 3' | -57.3 | NC_005045.1 | + | 35996 | 0.73 | 0.220999 |
Target: 5'- uUGGCGGCGuCGAGUgcuUCcUGUGCCa -3' miRNA: 3'- uGCCGCCGCuGCUCGac-AGcAUACGG- -5' |
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22375 | 3' | -57.3 | NC_005045.1 | + | 32484 | 0.72 | 0.232901 |
Target: 5'- cACGGCcuucGGCGACGAGCUGcacagccUGUcagGCCg -3' miRNA: 3'- -UGCCG----CCGCUGCUCGACa------GCAua-CGG- -5' |
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22375 | 3' | -57.3 | NC_005045.1 | + | 3681 | 0.72 | 0.245336 |
Target: 5'- gUGGcCGGUGACGAuGCUGUCGgcUaCCa -3' miRNA: 3'- uGCC-GCCGCUGCU-CGACAGCauAcGG- -5' |
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22375 | 3' | -57.3 | NC_005045.1 | + | 17408 | 0.71 | 0.285958 |
Target: 5'- uGCGGCGGCuGCGgcGGCUggaGUCGUggGCg -3' miRNA: 3'- -UGCCGCCGcUGC--UCGA---CAGCAuaCGg -5' |
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22375 | 3' | -57.3 | NC_005045.1 | + | 18271 | 0.71 | 0.285958 |
Target: 5'- cAUGGCGcuGCGGgcUGGGCUGUCG-AUGCUc -3' miRNA: 3'- -UGCCGC--CGCU--GCUCGACAGCaUACGG- -5' |
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22375 | 3' | -57.3 | NC_005045.1 | + | 31253 | 0.7 | 0.331701 |
Target: 5'- -aGGuUGGcCGACGAGCUGggCGUuacgaacaAUGCCu -3' miRNA: 3'- ugCC-GCC-GCUGCUCGACa-GCA--------UACGG- -5' |
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22375 | 3' | -57.3 | NC_005045.1 | + | 35495 | 0.69 | 0.382601 |
Target: 5'- cCGGUGGCGGCu-GCgggGUCGUc-GCCg -3' miRNA: 3'- uGCCGCCGCUGcuCGa--CAGCAuaCGG- -5' |
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22375 | 3' | -57.3 | NC_005045.1 | + | 37444 | 0.69 | 0.356513 |
Target: 5'- cCGGCGGCGGCGcucaaguaGGCgGUCuUGgucugGCCg -3' miRNA: 3'- uGCCGCCGCUGC--------UCGaCAGcAUa----CGG- -5' |
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22375 | 3' | -57.3 | NC_005045.1 | + | 30652 | 0.69 | 0.348099 |
Target: 5'- gGCGGCGGCGAUcuccagaaGGGCU-UCGcg-GCCc -3' miRNA: 3'- -UGCCGCCGCUG--------CUCGAcAGCauaCGG- -5' |
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22375 | 3' | -57.3 | NC_005045.1 | + | 12019 | 0.68 | 0.400684 |
Target: 5'- uACGGUGGCGAgGuGCgguacaaggGcCGgGUGCCg -3' miRNA: 3'- -UGCCGCCGCUgCuCGa--------CaGCaUACGG- -5' |
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22375 | 3' | -57.3 | NC_005045.1 | + | 12639 | 0.68 | 0.428808 |
Target: 5'- gACGGCGaCGGCGAcaacgcGuCUGUCGUccaGCCg -3' miRNA: 3'- -UGCCGCcGCUGCU------C-GACAGCAua-CGG- -5' |
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22375 | 3' | -57.3 | NC_005045.1 | + | 39001 | 0.68 | 0.438437 |
Target: 5'- gGCGGCGGUGAuccCGAuGCaGUUGaUAgagGCCg -3' miRNA: 3'- -UGCCGCCGCU---GCU-CGaCAGC-AUa--CGG- -5' |
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22375 | 3' | -57.3 | NC_005045.1 | + | 6464 | 0.67 | 0.509054 |
Target: 5'- gACGGCaagcgGGCGACcugggacaAGgUGgcggCGUGUGCCg -3' miRNA: 3'- -UGCCG-----CCGCUGc-------UCgACa---GCAUACGG- -5' |
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22375 | 3' | -57.3 | NC_005045.1 | + | 21641 | 0.67 | 0.509054 |
Target: 5'- gACGGCaGCGugGAGCaG-CGg--GCUa -3' miRNA: 3'- -UGCCGcCGCugCUCGaCaGCauaCGG- -5' |
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22375 | 3' | -57.3 | NC_005045.1 | + | 9663 | 0.67 | 0.498653 |
Target: 5'- -gGGCGGUaucuucaagGGCGuGCUGucccUCGUAggcUGCCg -3' miRNA: 3'- ugCCGCCG---------CUGCuCGAC----AGCAU---ACGG- -5' |
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22375 | 3' | -57.3 | NC_005045.1 | + | 6263 | 0.67 | 0.458062 |
Target: 5'- cCGGCGGCGucgUGGGCa---GUGUGCUg -3' miRNA: 3'- uGCCGCCGCu--GCUCGacagCAUACGG- -5' |
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22375 | 3' | -57.3 | NC_005045.1 | + | 15546 | 0.66 | 0.562281 |
Target: 5'- cAUGGCGuaGGCGuccaucaucauGCcGUCGUcgGCCu -3' miRNA: 3'- -UGCCGCcgCUGCu----------CGaCAGCAuaCGG- -5' |
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22375 | 3' | -57.3 | NC_005045.1 | + | 18897 | 0.66 | 0.540771 |
Target: 5'- cUGGCccaGGUGGCgGAGCUG-CGccUGCCg -3' miRNA: 3'- uGCCG---CCGCUG-CUCGACaGCauACGG- -5' |
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22375 | 3' | -57.3 | NC_005045.1 | + | 41505 | 0.66 | 0.530119 |
Target: 5'- uCGuGUagGGCGAUGcGGUUGUCG-GUGCCg -3' miRNA: 3'- uGC-CG--CCGCUGC-UCGACAGCaUACGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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