Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 22376 | 3' | -56.3 | NC_005045.1 | + | 27461 | 0.71 | 0.292275 |
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Target: 5'- gCCU-CCAGCggcuaccugguguuuGGUACCagcaCGGCGGACGAGa -3' miRNA: 3'- -GGAcGGUCG---------------UCAUGG----GUCGCCUGUUC- -5' |
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| 22376 | 3' | -56.3 | NC_005045.1 | + | 5232 | 0.72 | 0.27485 |
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Target: 5'- gCUGCCcuGGCcguGUGCCagCAGCGGGCAGa -3' miRNA: 3'- gGACGG--UCGu--CAUGG--GUCGCCUGUUc -5' |
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| 22376 | 3' | -56.3 | NC_005045.1 | + | 7514 | 0.73 | 0.241235 |
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Target: 5'- uCCUGacaggagUAGCAGUaugccACCCAGCGGuCAGGa -3' miRNA: 3'- -GGACg------GUCGUCA-----UGGGUCGCCuGUUC- -5' |
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| 22376 | 3' | -56.3 | NC_005045.1 | + | 27509 | 0.73 | 0.234937 |
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Target: 5'- aUCUGCCAcCAGUACCUcuGGCaGGGCAAc -3' miRNA: 3'- -GGACGGUcGUCAUGGG--UCG-CCUGUUc -5' |
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| 22376 | 3' | -56.3 | NC_005045.1 | + | 11239 | 0.73 | 0.211117 |
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Target: 5'- -aUGCCAucCAGUACCCGGCGGGgAAu -3' miRNA: 3'- ggACGGUc-GUCAUGGGUCGCCUgUUc -5' |
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| 22376 | 3' | -56.3 | NC_005045.1 | + | 37555 | 0.74 | 0.205496 |
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Target: 5'- uCCUGCCGG-AGUcuCCCAGCGGAgGu- -3' miRNA: 3'- -GGACGGUCgUCAu-GGGUCGCCUgUuc -5' |
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| 22376 | 3' | -56.3 | NC_005045.1 | + | 30579 | 0.74 | 0.188889 |
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Target: 5'- gCCaGCCAGUucgccucgucgauGGUcGCCCGGCGGACAu- -3' miRNA: 3'- -GGaCGGUCG-------------UCA-UGGGUCGCCUGUuc -5' |
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| 22376 | 3' | -56.3 | NC_005045.1 | + | 14827 | 0.87 | 0.02257 |
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Target: 5'- aCCUGCCGGCAGUGCCCaacgucgcGGCGGAUg-- -3' miRNA: 3'- -GGACGGUCGUCAUGGG--------UCGCCUGuuc -5' |
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| 22376 | 3' | -56.3 | NC_005045.1 | + | 40012 | 1.09 | 0.000511 |
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Target: 5'- uCCUGCCAGCAGUACCCAGCGGACAAGc -3' miRNA: 3'- -GGACGGUCGUCAUGGGUCGCCUGUUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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