Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 22376 | 3' | -56.3 | NC_005045.1 | + | 9468 | 0.7 | 0.328057 |
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Target: 5'- cCCgGUCGGCAGUgcaauggaACCCAGUGGAa--- -3' miRNA: 3'- -GGaCGGUCGUCA--------UGGGUCGCCUguuc -5' |
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| 22376 | 3' | -56.3 | NC_005045.1 | + | 24060 | 0.7 | 0.328057 |
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Target: 5'- aCCUccGCuCGGUAuUGCUCAGCGGACAGa -3' miRNA: 3'- -GGA--CG-GUCGUcAUGGGUCGCCUGUUc -5' |
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| 22376 | 3' | -56.3 | NC_005045.1 | + | 4123 | 0.71 | 0.312113 |
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Target: 5'- gCCUGCCAGCAG-GCCgaggCAGCaGuACAGGc -3' miRNA: 3'- -GGACGGUCGUCaUGG----GUCGcC-UGUUC- -5' |
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| 22376 | 3' | -56.3 | NC_005045.1 | + | 11003 | 0.71 | 0.296764 |
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Target: 5'- aUCUGCCAGCAGg---CAGCGGAUc-- -3' miRNA: 3'- -GGACGGUCGUCauggGUCGCCUGuuc -5' |
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| 22376 | 3' | -56.3 | NC_005045.1 | + | 7514 | 0.73 | 0.241235 |
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Target: 5'- uCCUGacaggagUAGCAGUaugccACCCAGCGGuCAGGa -3' miRNA: 3'- -GGACg------GUCGUCA-----UGGGUCGCCuGUUC- -5' |
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| 22376 | 3' | -56.3 | NC_005045.1 | + | 11239 | 0.73 | 0.211117 |
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Target: 5'- -aUGCCAucCAGUACCCGGCGGGgAAu -3' miRNA: 3'- ggACGGUc-GUCAUGGGUCGCCUgUUc -5' |
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| 22376 | 3' | -56.3 | NC_005045.1 | + | 37555 | 0.74 | 0.205496 |
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Target: 5'- uCCUGCCGG-AGUcuCCCAGCGGAgGu- -3' miRNA: 3'- -GGACGGUCgUCAu-GGGUCGCCUgUuc -5' |
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| 22376 | 3' | -56.3 | NC_005045.1 | + | 30579 | 0.74 | 0.188889 |
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Target: 5'- gCCaGCCAGUucgccucgucgauGGUcGCCCGGCGGACAu- -3' miRNA: 3'- -GGaCGGUCG-------------UCA-UGGGUCGCCUGUuc -5' |
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| 22376 | 3' | -56.3 | NC_005045.1 | + | 13247 | 0.66 | 0.5948 |
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Target: 5'- gCCUGCCGGCaucuGGUcCUCGGCcuuguacauGACGAGg -3' miRNA: 3'- -GGACGGUCG----UCAuGGGUCGc--------CUGUUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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