Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 22376 | 3' | -56.3 | NC_005045.1 | + | 14125 | 0.69 | 0.416562 |
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Target: 5'- -gUGgCGGCGGUcgcaucuuCCCGGCGGGCAc- -3' miRNA: 3'- ggACgGUCGUCAu-------GGGUCGCCUGUuc -5' |
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| 22376 | 3' | -56.3 | NC_005045.1 | + | 13194 | 0.68 | 0.426185 |
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Target: 5'- -aUGCCGGCAGgcGCCUucAGCGaGAUggGg -3' miRNA: 3'- ggACGGUCGUCa-UGGG--UCGC-CUGuuC- -5' |
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| 22376 | 3' | -56.3 | NC_005045.1 | + | 6013 | 0.68 | 0.435938 |
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Target: 5'- --aGCCucaAGCAGggcuugACCCGGCuGGugGAGg -3' miRNA: 3'- ggaCGG---UCGUCa-----UGGGUCG-CCugUUC- -5' |
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| 22376 | 3' | -56.3 | NC_005045.1 | + | 11544 | 0.68 | 0.435938 |
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Target: 5'- gCCggGUCuGguGUACCCAGCaGGcCGAGu -3' miRNA: 3'- -GGa-CGGuCguCAUGGGUCG-CCuGUUC- -5' |
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| 22376 | 3' | -56.3 | NC_005045.1 | + | 8640 | 0.68 | 0.455824 |
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Target: 5'- gCUGgUGGCAGcGCCUccugauGCGGGCAAGa -3' miRNA: 3'- gGACgGUCGUCaUGGGu-----CGCCUGUUC- -5' |
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| 22376 | 3' | -56.3 | NC_005045.1 | + | 19570 | 0.66 | 0.550647 |
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Target: 5'- aCCUGCucaggacucCAGCAcUACUCGGCuauucuccgGGACGAGa -3' miRNA: 3'- -GGACG---------GUCGUcAUGGGUCG---------CCUGUUC- -5' |
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| 22376 | 3' | -56.3 | NC_005045.1 | + | 16876 | 0.66 | 0.572623 |
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Target: 5'- aCUGCCAG-GGcGCCCcGCuGGACGAc -3' miRNA: 3'- gGACGGUCgUCaUGGGuCG-CCUGUUc -5' |
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| 22376 | 3' | -56.3 | NC_005045.1 | + | 39631 | 0.66 | 0.5948 |
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Target: 5'- cCCUGCCGGCGa----CGGCGGcgACGAGc -3' miRNA: 3'- -GGACGGUCGUcauggGUCGCC--UGUUC- -5' |
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| 22376 | 3' | -56.3 | NC_005045.1 | + | 40012 | 1.09 | 0.000511 |
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Target: 5'- uCCUGCCAGCAGUACCCAGCGGACAAGc -3' miRNA: 3'- -GGACGGUCGUCAUGGGUCGCCUGUUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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