Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 22379 | 5' | -57.7 | NC_005045.1 | + | 2908 | 0.66 | 0.491295 |
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Target: 5'- -gCUGCGUUGcAGCGGCCcgcCGGGu -3' miRNA: 3'- uaGGCGCGACaUCGCCGGauaGUCCu -5' |
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| 22379 | 5' | -57.7 | NC_005045.1 | + | 13886 | 0.66 | 0.440324 |
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Target: 5'- -gCCGCGCUGccguccgGGUGGUCcacgaacGUCAGGGu -3' miRNA: 3'- uaGGCGCGACa------UCGCCGGa------UAGUCCU- -5' |
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| 22379 | 5' | -57.7 | NC_005045.1 | + | 33131 | 0.68 | 0.36547 |
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Target: 5'- -aCCGuCGCUGUAGCgcaGGCgguUCAGGGu -3' miRNA: 3'- uaGGC-GCGACAUCG---CCGgauAGUCCU- -5' |
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| 22379 | 5' | -57.7 | NC_005045.1 | + | 30734 | 0.68 | 0.348179 |
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Target: 5'- --gCGCGCUGUcgcGGCGGCUgaaCAGGu -3' miRNA: 3'- uagGCGCGACA---UCGCCGGauaGUCCu -5' |
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| 22379 | 5' | -57.7 | NC_005045.1 | + | 23873 | 0.69 | 0.315377 |
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Target: 5'- gGUCCGCGUguugGUugaagacgccGGCaGCCUGcuUCAGGAc -3' miRNA: 3'- -UAGGCGCGa---CA----------UCGcCGGAU--AGUCCU- -5' |
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| 22379 | 5' | -57.7 | NC_005045.1 | + | 9270 | 0.7 | 0.270646 |
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Target: 5'- gAUCCGCaccGUUGaGGCGGCCUAgguGGAu -3' miRNA: 3'- -UAGGCG---CGACaUCGCCGGAUaguCCU- -5' |
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| 22379 | 5' | -57.7 | NC_005045.1 | + | 41295 | 1.06 | 0.00051 |
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Target: 5'- gAUCCGCGCUGUAGCGGCCUAUCAGGAc -3' miRNA: 3'- -UAGGCGCGACAUCGCCGGAUAGUCCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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