Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 22398 | 3' | -55.9 | NC_005056.1 | + | 20083 | 0.66 | 0.562986 |
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Target: 5'- gGCAacGGUucaGGGUGUCCugGGugGCUUugACCGg -3' miRNA: 3'- -CGU--CCG---CCCAUAGGugUC--UGAG--UGGC- -5' |
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| 22398 | 3' | -55.9 | NC_005056.1 | + | 2228 | 0.66 | 0.562986 |
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Target: 5'- aGguGGCaucaGGUAUggccUCACAGAgUCACCu -3' miRNA: 3'- -CguCCGc---CCAUA----GGUGUCUgAGUGGc -5' |
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| 22398 | 3' | -55.9 | NC_005056.1 | + | 11464 | 0.67 | 0.486808 |
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Target: 5'- gGCAGGUguccGGGU-UCCACAG-UUCACg- -3' miRNA: 3'- -CGUCCG----CCCAuAGGUGUCuGAGUGgc -5' |
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| 22398 | 3' | -55.9 | NC_005056.1 | + | 16013 | 0.71 | 0.287199 |
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Target: 5'- cGCuGGCGGcGUcgUUGCAGGCuaUCGCCGa -3' miRNA: 3'- -CGuCCGCC-CAuaGGUGUCUG--AGUGGC- -5' |
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| 22398 | 3' | -55.9 | NC_005056.1 | + | 1664 | 0.75 | 0.149168 |
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Target: 5'- uCAGGCGGaauccAUCCGCAaACUCACCGa -3' miRNA: 3'- cGUCCGCCca---UAGGUGUcUGAGUGGC- -5' |
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| 22398 | 3' | -55.9 | NC_005056.1 | + | 5630 | 1.12 | 0.000291 |
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Target: 5'- aGCAGGCGGGUAUCCACAGACUCACCGg -3' miRNA: 3'- -CGUCCGCCCAUAGGUGUCUGAGUGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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