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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 22403 | 3' | -56.3 | NC_005056.1 | + | 2960 | 0.66 | 0.481248 |
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Target: 5'- uGGCAGGGGuuuuccgUGCCgCAGAUugccgccauGUUGCaGAUg -3' miRNA: 3'- gCCGUCCCU-------GCGG-GUCUA---------UAACG-CUG- -5' |
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| 22403 | 3' | -56.3 | NC_005056.1 | + | 2029 | 0.67 | 0.408453 |
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Target: 5'- gCGGCAGauugucaccauugaGGACGCCCuGAaag-GUGGCu -3' miRNA: 3'- -GCCGUC--------------CCUGCGGGuCUauaaCGCUG- -5' |
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| 22403 | 3' | -56.3 | NC_005056.1 | + | 3358 | 0.7 | 0.275789 |
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Target: 5'- aGGCA-GGAUGCCgcgCAGAUGUuccagguuaaUGCGGCa -3' miRNA: 3'- gCCGUcCCUGCGG---GUCUAUA----------ACGCUG- -5' |
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| 22403 | 3' | -56.3 | NC_005056.1 | + | 667 | 0.7 | 0.26862 |
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Target: 5'- aGGCAGaguuaaGGGCGCUgAGAUAUUcCGGCa -3' miRNA: 3'- gCCGUC------CCUGCGGgUCUAUAAcGCUG- -5' |
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| 22403 | 3' | -56.3 | NC_005056.1 | + | 7440 | 1.1 | 0.000292 |
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Target: 5'- cCGGCAGGGACGCCCAGAUAUUGCGACa -3' miRNA: 3'- -GCCGUCCCUGCGGGUCUAUAACGCUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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