Results 1 - 9 of 9 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22405 | 3' | -47.2 | NC_005056.1 | + | 11056 | 0.66 | 0.944439 |
Target: 5'- gUCacgCGGUCGgccaccggcCUG-ACGUCCUCACCa -3' miRNA: 3'- -AGaa-GUUAGUa--------GACgUGUAGGAGUGG- -5' |
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22405 | 3' | -47.2 | NC_005056.1 | + | 3854 | 0.66 | 0.944439 |
Target: 5'- --cUCAGUUuuucCUGCACAUgCUCAgCg -3' miRNA: 3'- agaAGUUAGua--GACGUGUAgGAGUgG- -5' |
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22405 | 3' | -47.2 | NC_005056.1 | + | 7277 | 0.68 | 0.906625 |
Target: 5'- aUUUCAGUCcgUUGuCACAggacagaCUCGCCa -3' miRNA: 3'- aGAAGUUAGuaGAC-GUGUag-----GAGUGG- -5' |
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22405 | 3' | -47.2 | NC_005056.1 | + | 14345 | 0.68 | 0.874812 |
Target: 5'- aCUcCAGUCG--UGCACGUuCCUCAUCc -3' miRNA: 3'- aGAaGUUAGUagACGUGUA-GGAGUGG- -5' |
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22405 | 3' | -47.2 | NC_005056.1 | + | 4776 | 0.69 | 0.866077 |
Target: 5'- --cUCAAUCAggUUGUuggucGCAUCCaUCACCa -3' miRNA: 3'- agaAGUUAGUa-GACG-----UGUAGG-AGUGG- -5' |
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22405 | 3' | -47.2 | NC_005056.1 | + | 3153 | 0.69 | 0.857046 |
Target: 5'- --aUCAGUUucccgacgaUGCGCAUCCUcCGCCa -3' miRNA: 3'- agaAGUUAGuag------ACGUGUAGGA-GUGG- -5' |
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22405 | 3' | -47.2 | NC_005056.1 | + | 9139 | 0.72 | 0.706414 |
Target: 5'- ---cCAGUCGUUuuuUGCAUAUCCgCGCCa -3' miRNA: 3'- agaaGUUAGUAG---ACGUGUAGGaGUGG- -5' |
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22405 | 3' | -47.2 | NC_005056.1 | + | 9270 | 0.74 | 0.549806 |
Target: 5'- --aUUGAUUAUCcgGCGCGUCCUCugCu -3' miRNA: 3'- agaAGUUAGUAGa-CGUGUAGGAGugG- -5' |
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22405 | 3' | -47.2 | NC_005056.1 | + | 9364 | 1.15 | 0.001413 |
Target: 5'- cUCUUCAAUCAUCUGCACAUCCUCACCg -3' miRNA: 3'- -AGAAGUUAGUAGACGUGUAGGAGUGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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