Results 1 - 11 of 11 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22408 | 5' | -57 | NC_005056.1 | + | 12706 | 0.66 | 0.407606 |
Target: 5'- gAUUGCCGCCCuUGUAGaCUCG-UCGCc -3' miRNA: 3'- -UAGUGGCGGGcACGUCgGAGUaAGUG- -5' |
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22408 | 5' | -57 | NC_005056.1 | + | 166 | 0.67 | 0.388486 |
Target: 5'- --aGCCGCCCGcagGCGGCgaUGUUCAg -3' miRNA: 3'- uagUGGCGGGCa--CGUCGgaGUAAGUg -5' |
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22408 | 5' | -57 | NC_005056.1 | + | 12232 | 0.67 | 0.388486 |
Target: 5'- cAUCAUCGUCagcaGUGUGGCCUCAguggaGCu -3' miRNA: 3'- -UAGUGGCGGg---CACGUCGGAGUaag--UG- -5' |
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22408 | 5' | -57 | NC_005056.1 | + | 8855 | 0.67 | 0.360932 |
Target: 5'- gGUCAcCCGCCCgGUGgAGCUUUAUaUCAa -3' miRNA: 3'- -UAGU-GGCGGG-CACgUCGGAGUA-AGUg -5' |
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22408 | 5' | -57 | NC_005056.1 | + | 3542 | 0.68 | 0.318113 |
Target: 5'- cGUCACCGaugaCCCG-GCaAGCCUCgg-CACg -3' miRNA: 3'- -UAGUGGC----GGGCaCG-UCGGAGuaaGUG- -5' |
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22408 | 5' | -57 | NC_005056.1 | + | 9516 | 0.68 | 0.294311 |
Target: 5'- gGUCGCUGCCgUGUGCAGaCCggcggCAUgugCACc -3' miRNA: 3'- -UAGUGGCGG-GCACGUC-GGa----GUAa--GUG- -5' |
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22408 | 5' | -57 | NC_005056.1 | + | 16778 | 0.7 | 0.250918 |
Target: 5'- -cCACCGCCCacauGUGC-GCCUCAgcCAUc -3' miRNA: 3'- uaGUGGCGGG----CACGuCGGAGUaaGUG- -5' |
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22408 | 5' | -57 | NC_005056.1 | + | 16439 | 0.7 | 0.237672 |
Target: 5'- -cCACCGCCCGUGC-GCUgggUCug-CGCg -3' miRNA: 3'- uaGUGGCGGGCACGuCGG---AGuaaGUG- -5' |
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22408 | 5' | -57 | NC_005056.1 | + | 27841 | 0.7 | 0.224399 |
Target: 5'- aAUCACCacugucugcggcgGCgCGUGCAGCCUCGa-CGCg -3' miRNA: 3'- -UAGUGG-------------CGgGCACGUCGGAGUaaGUG- -5' |
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22408 | 5' | -57 | NC_005056.1 | + | 218 | 0.71 | 0.20711 |
Target: 5'- cAUCGCCGCCUGcggGCGGCUUU-UUUACa -3' miRNA: 3'- -UAGUGGCGGGCa--CGUCGGAGuAAGUG- -5' |
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22408 | 5' | -57 | NC_005056.1 | + | 10680 | 1.08 | 0.000305 |
Target: 5'- cAUCACCGCCCGUGCAGCCUCAUUCACa -3' miRNA: 3'- -UAGUGGCGGGCACGUCGGAGUAAGUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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