Results 1 - 12 of 12 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22410 | 3' | -54.1 | NC_005056.1 | + | 2524 | 0.66 | 0.62199 |
Target: 5'- -cCGCCAGaUUGGCGcgACGUUuuauuuUUCCCg -3' miRNA: 3'- caGCGGUCcGACCGU--UGUAGu-----AAGGG- -5' |
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22410 | 3' | -54.1 | NC_005056.1 | + | 2984 | 0.66 | 0.60922 |
Target: 5'- cGUCGUCAGGCggcgcugcuguacUGGCAGgGgu-UUUCCg -3' miRNA: 3'- -CAGCGGUCCG-------------ACCGUUgUaguAAGGG- -5' |
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22410 | 3' | -54.1 | NC_005056.1 | + | 4744 | 0.66 | 0.62199 |
Target: 5'- -gUGCUcgAGGCUGGCAuaAUCAccCCCc -3' miRNA: 3'- caGCGG--UCCGACCGUugUAGUaaGGG- -5' |
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22410 | 3' | -54.1 | NC_005056.1 | + | 8038 | 0.68 | 0.475442 |
Target: 5'- -gCGCgCAGGCUGGCGuuuUCAUgcuUCgCCa -3' miRNA: 3'- caGCG-GUCCGACCGUuguAGUA---AG-GG- -5' |
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22410 | 3' | -54.1 | NC_005056.1 | + | 8547 | 0.73 | 0.260886 |
Target: 5'- aUUGCCuccacGCUGGCGACGUCGUUgUCa -3' miRNA: 3'- cAGCGGuc---CGACCGUUGUAGUAAgGG- -5' |
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22410 | 3' | -54.1 | NC_005056.1 | + | 8599 | 0.68 | 0.486192 |
Target: 5'- cGUCGCCAGcGUggaGGCAAUAc---UCCCg -3' miRNA: 3'- -CAGCGGUC-CGa--CCGUUGUaguaAGGG- -5' |
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22410 | 3' | -54.1 | NC_005056.1 | + | 9393 | 0.71 | 0.322413 |
Target: 5'- -cCGCCGGuaUgacccGGCGGCAUCcUUCCCg -3' miRNA: 3'- caGCGGUCcgA-----CCGUUGUAGuAAGGG- -5' |
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22410 | 3' | -54.1 | NC_005056.1 | + | 11444 | 0.69 | 0.443931 |
Target: 5'- -cUGCCGuGGCUGGCGugGgCGUUUUCg -3' miRNA: 3'- caGCGGU-CCGACCGUugUaGUAAGGG- -5' |
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22410 | 3' | -54.1 | NC_005056.1 | + | 12953 | 1.11 | 0.000437 |
Target: 5'- gGUCGCCAGGCUGGCAACAUCAUUCCCc -3' miRNA: 3'- -CAGCGGUCCGACCGUUGUAGUAAGGG- -5' |
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22410 | 3' | -54.1 | NC_005056.1 | + | 15996 | 0.68 | 0.475442 |
Target: 5'- --gGCCGGuaaaaaaggcacGCUGGCGGCGUCGUUgCa -3' miRNA: 3'- cagCGGUC------------CGACCGUUGUAGUAAgGg -5' |
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22410 | 3' | -54.1 | NC_005056.1 | + | 20283 | 0.66 | 0.60922 |
Target: 5'- gGUCGCCGgaauauaaccaccGGUgccauacgUGGCGGCAUUAgUCCUu -3' miRNA: 3'- -CAGCGGU-------------CCG--------ACCGUUGUAGUaAGGG- -5' |
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22410 | 3' | -54.1 | NC_005056.1 | + | 20721 | 0.66 | 0.598791 |
Target: 5'- --aGCCuGaGCgUGGCAuCGUCAgcUUCCCg -3' miRNA: 3'- cagCGGuC-CG-ACCGUuGUAGU--AAGGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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