Results 21 - 25 of 25 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22414 | 3' | -58.9 | NC_005056.1 | + | 11775 | 0.71 | 0.17526 |
Target: 5'- gGUGGCGGCUugCAgguuUGGCAucagcaauCAGCCGu -3' miRNA: 3'- -UACCGCCGGugGU----GCCGUu-------GUUGGCc -5' |
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22414 | 3' | -58.9 | NC_005056.1 | + | 19775 | 0.71 | 0.161271 |
Target: 5'- uAUGaCGGCCAUCGgGGCGAUuagcuGGCCGGu -3' miRNA: 3'- -UACcGCCGGUGGUgCCGUUG-----UUGGCC- -5' |
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22414 | 3' | -58.9 | NC_005056.1 | + | 12758 | 0.75 | 0.081211 |
Target: 5'- cUGGCGGCgGCgCugGGCAAUGACCc- -3' miRNA: 3'- uACCGCCGgUG-GugCCGUUGUUGGcc -5' |
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22414 | 3' | -58.9 | NC_005056.1 | + | 9584 | 0.76 | 0.072245 |
Target: 5'- -cGGUGcacauGCCGCCggucugcacACGGCAGCGACCGGc -3' miRNA: 3'- uaCCGC-----CGGUGG---------UGCCGUUGUUGGCC- -5' |
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22414 | 3' | -58.9 | NC_005056.1 | + | 19860 | 1.07 | 0.000285 |
Target: 5'- aAUGGCGGCCACCACGGCAACAACCGGc -3' miRNA: 3'- -UACCGCCGGUGGUGCCGUUGUUGGCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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