Results 1 - 11 of 11 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
22431 | 3' | -65.5 | NC_005056.1 | + | 6020 | 0.66 | 0.18907 |
Target: 5'- cUCUGaaaaGUC-GGGCGGUggACGCCGGUaCAg -3' miRNA: 3'- -GGGCg---CGGuCCCGCCG--UGCGGCCA-GU- -5' |
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22431 | 3' | -65.5 | NC_005056.1 | + | 10407 | 0.66 | 0.18907 |
Target: 5'- gCUG-GCCGGuaucGGUGuGCugGCCGGUUAa -3' miRNA: 3'- gGGCgCGGUC----CCGC-CGugCGGCCAGU- -5' |
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22431 | 3' | -65.5 | NC_005056.1 | + | 20265 | 0.66 | 0.179472 |
Target: 5'- aCCgGUGCCAuacGuGGCGGCauuaguccuuGCGgCGGUCu -3' miRNA: 3'- -GGgCGCGGU---C-CCGCCG----------UGCgGCCAGu -5' |
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22431 | 3' | -65.5 | NC_005056.1 | + | 10021 | 0.66 | 0.170308 |
Target: 5'- cCCCgGCGaCAGcGGCGGCACaaCCGGg-- -3' miRNA: 3'- -GGG-CGCgGUC-CCGCCGUGc-GGCCagu -5' |
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22431 | 3' | -65.5 | NC_005056.1 | + | 2989 | 0.66 | 0.165885 |
Target: 5'- aCCCGCGUCGucaGGCGGCGCuGCUGu--- -3' miRNA: 3'- -GGGCGCGGUc--CCGCCGUG-CGGCcagu -5' |
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22431 | 3' | -65.5 | NC_005056.1 | + | 10590 | 0.67 | 0.149208 |
Target: 5'- uUCCaGCGUCAGGGUacgGGCGaccgccuccUGCUGGUCu -3' miRNA: 3'- -GGG-CGCGGUCCCG---CCGU---------GCGGCCAGu -5' |
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22431 | 3' | -65.5 | NC_005056.1 | + | 18533 | 0.68 | 0.134071 |
Target: 5'- -aCGCGCCAGcGCGuCACgcuGCCGGUUAa -3' miRNA: 3'- ggGCGCGGUCcCGCcGUG---CGGCCAGU- -5' |
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22431 | 3' | -65.5 | NC_005056.1 | + | 11910 | 0.68 | 0.12366 |
Target: 5'- aCCGCGCCGGGaG-GGCG-GaaGGUCAa -3' miRNA: 3'- gGGCGCGGUCC-CgCCGUgCggCCAGU- -5' |
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22431 | 3' | -65.5 | NC_005056.1 | + | 10264 | 0.68 | 0.117141 |
Target: 5'- --aGCGCCGGGGUcuGCG-GCUGGUCAa -3' miRNA: 3'- gggCGCGGUCCCGc-CGUgCGGCCAGU- -5' |
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22431 | 3' | -65.5 | NC_005056.1 | + | 18823 | 0.73 | 0.046884 |
Target: 5'- gCUGCgGCUAuucaGGCGGCaACGCCGGUCAc -3' miRNA: 3'- gGGCG-CGGUc---CCGCCG-UGCGGCCAGU- -5' |
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22431 | 3' | -65.5 | NC_005056.1 | + | 5909 | 1.09 | 5.7e-05 |
Target: 5'- cCCCGCGCCAGGGCGGCACGCCGGUCAg -3' miRNA: 3'- -GGGCGCGGUCCCGCCGUGCGGCCAGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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