Results 21 - 25 of 25 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 22442 | 3' | -56.2 | NC_005056.1 | + | 11939 | 0.73 | 0.180452 |
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Target: 5'- gCCGCuGcCAGCUUuacugcACCGGCAGUgGUGAUa -3' miRNA: 3'- -GGCG-C-GUCGAAu-----UGGCCGUCG-CACUG- -5' |
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| 22442 | 3' | -56.2 | NC_005056.1 | + | 17173 | 0.74 | 0.159868 |
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Target: 5'- aCCGCGaUAGUUUucgAGCUuacgggucaguuuuGGCAGCGUGACg -3' miRNA: 3'- -GGCGC-GUCGAA---UUGG--------------CCGUCGCACUG- -5' |
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| 22442 | 3' | -56.2 | NC_005056.1 | + | 3965 | 0.76 | 0.121031 |
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Target: 5'- gCGcCGCAGCaaagccACCGGCgguGGCGUGACg -3' miRNA: 3'- gGC-GCGUCGaau---UGGCCG---UCGCACUG- -5' |
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| 22442 | 3' | -56.2 | NC_005056.1 | + | 17732 | 0.82 | 0.040199 |
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Target: 5'- aCCGCGCAGCguc-CCGGCuuggguuucaucAGCGUGACc -3' miRNA: 3'- -GGCGCGUCGaauuGGCCG------------UCGCACUG- -5' |
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| 22442 | 3' | -56.2 | NC_005056.1 | + | 18463 | 1.12 | 0.000219 |
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Target: 5'- aCCGCGCAGCUUAACCGGCAGCGUGACg -3' miRNA: 3'- -GGCGCGUCGAAUUGGCCGUCGCACUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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