Results 21 - 25 of 25 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 22442 | 3' | -56.2 | NC_005056.1 | + | 21200 | 0.67 | 0.414901 |
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Target: 5'- aCCGUcagGaCAGCgccacgAACCGGCAGCccGACc -3' miRNA: 3'- -GGCG---C-GUCGaa----UUGGCCGUCGcaCUG- -5' |
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| 22442 | 3' | -56.2 | NC_005056.1 | + | 20163 | 0.66 | 0.475541 |
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Target: 5'- aCUGCGCAGUuuguugaacgcaUUAAgCGGCAGCa---- -3' miRNA: 3'- -GGCGCGUCG------------AAUUgGCCGUCGcacug -5' |
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| 22442 | 3' | -56.2 | NC_005056.1 | + | 8900 | 0.66 | 0.475541 |
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Target: 5'- aCCGgGCGGg-UGACCGGCuccGGCGgcgGGg -3' miRNA: 3'- -GGCgCGUCgaAUUGGCCG---UCGCa--CUg -5' |
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| 22442 | 3' | -56.2 | NC_005056.1 | + | 2910 | 0.66 | 0.507474 |
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Target: 5'- cCCGaCGguGCagAGCUGGaaaCAGCGcGACg -3' miRNA: 3'- -GGC-GCguCGaaUUGGCC---GUCGCaCUG- -5' |
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| 22442 | 3' | -56.2 | NC_005056.1 | + | 9574 | 0.66 | 0.529259 |
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Target: 5'- gCCGC-CGGuCUgcACaCGGCAGCGaccGGCg -3' miRNA: 3'- -GGCGcGUC-GAauUG-GCCGUCGCa--CUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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